What is the evolution of plasma LPS in alcohol withdrawal?
Levels of Plasma Apolipoproteins in healthy subjects
27 November 2023 6,448 0 View
Hello! I am trying to isolate enteric fibroblasts from the small intestine and colon of mice. I am successful in isolating fibroblasts from the colon, but not from the small intestine following...
21 February 2021 6,389 5 View
I’m researching the biosorption of lead in aqueous solution onto industrial wastes of potato peel modified with citric acid. I want to use the Response Surface Methodology (RSM) to optimize the...
12 January 2021 832 4 View
Of course I will cite you properly, just let me know if you are OK with that, Carlos
27 May 2019 4,279 0 View
We are interested in measuring NOy during field campaigns in order to analyse the behaviour of O3. We know there are commercial monitors based on chemiluminiscence and we would be wondering...
06 February 2018 1,723 0 View
Hello! I work with mouse islets and I want to detect phospho-Insulin Receptor by western blot but all the antibodies I have been testing are horrible... even with amounts of protein around...
25 January 2018 9,279 2 View
Hello! I am trying to stimulate the insulin receptor in mouse islets by the incubation with insulin (10nM) for 5 minuts in order to find a deffect in the signaling. So then I perform a Western...
25 January 2018 4,634 4 View
It seems that people got them as gifts from collaborators, but not in a comercial distributor...all info is welcome :)
17 January 2018 5,467 2 View
I am currently trying to find ways to extract, as automatically as possible, bibliographic entries from accessible, editable (i.e. not image files) PDF files, in any format. My next move would be...
10 October 2017 9,757 4 View
I am gonna start working with this thyroid cell line and I was wondering if anybody knows if they are "easy" for tansfection,growth and so on. In addition, do you used siRNAs with them? Thanks to...
21 September 2016 2,645 1 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.
07 August 2024 4,526 4 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
In my experiment, I treated RAW macrophages with 100 ng/mL of LPS. After 24 hours, I collected the supernatant and replaced it with fresh media. I then quantified the cytokine levels of TNF and...
01 August 2024 476 2 View
or can you recommend articles showing the differential effect of LPS in a time dependant manner?
28 July 2024 2,547 1 View
I am using the Polyomics Integrated Metabolomics Pipeline (PiMP) for metabolite feature analysis. After annotating my data, I have encountered cases where multiple potential metabolites are...
24 July 2024 5,789 0 View
I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...
22 July 2024 9,160 2 View
Is there a method that I can measure alcohol dehydrogenase activity using NAD+ in enzyme extraction from plants?
17 July 2024 935 1 View
Hi everybody, I wonder is there a way to prepare foam from iso-propanol/water mixture? The content of this alcohol should be 70 – 80 w.%. I plan to use silicone-based surfactants, I have several...
15 July 2024 3,539 0 View
Hello, I am an Etch Engineer. I am performing etching with high T_ox/Nit selectivity using remote plasma. Etching plasma is formed using only NH3/NF3/H2. 1. I wonder if it would be advantageous...
11 July 2024 8,115 2 View
