Are ZnS nanoparticles moisture sensitive?

Hello Everyone, I tried to form ZnS nanoparticles using the hot injection method and was able to form the same (confirmed by XRD). When I centrifuged, after the reaction I got a kind of white...

17 February 2021 1,709 3 View

Which kind of validity should e established in case we want to conduct a survey using newly developed survey questionnaire?

I am conducting a survey using self developed questionnaire. I have established its face validity and content validity. Some expert reviewer has suggested I must establish the validity by...

11 February 2021 2,077 3 View

How to calculate width and length of the tapered microstrip line feed of a SIW leaky wave antenna?

With reference to substrate integrated waveguide -leaky wave antenna

26 December 2020 3,982 2 View

Sample does no retain in the well while doing NATIVE Gel electrophoresis?

While doing NATIVE-Gel electrophoresis with electrophoresis buffer containing 25mM Tris, 192 mM glycine, and rest water if the sample being loaded is dissolved in methanol it won't remain in the...

15 December 2020 4,999 3 View

How to identify and quantify glauconite and illite/other mica present in same sample from XRD analysis?

In glauconite bearing sand stone sample other mica group minerals(illite, muscovite etc.) are also present. I want to quantify the amount of glauconite and othermica group minerals. How, I can...

10 December 2020 5,793 19 View

Collaborating VS contributing Authorship?

Can you help me to understand the significance of the difference between collaborating and contributing authorship (eg, GBD)? Do they put similar weightage to University applicant assessors?

29 October 2020 4,443 3 View

What will be the impact on Socio-Economic activities of man after the end of COVID-19 Pandemic?

I am very much thankful of the experts, researcher, and thinkers for very thought full discussion on my previous question on Role of Geography on COVID-19 Pandemic. Now I am keeping another very...

19 October 2020 6,552 4 View

How to model deformable metal powder?? How to define gap between the particles?? Which modeling software can be used??

We need to model deformable metal powder. It will be further used in simulation process.

30 September 2020 9,329 7 View

How to prevent leaks in Swagelok fittings?

I am carrying out a liquefaction reaction inside a 2.5mL volume reactor made of Swagelok fittings (one SS 3/4" STRAIGHT UNION with two SS 3/4" PLUGS FOR FITTING). After adding the substrate...

11 August 2020 4,211 2 View

How to prevent lignin condensation during biomass fractionation?

10 August 2020 4,936 1 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View