Hi,

In addition to the HPLC, you obviously can use High-Performance Thin-Layer Chromatography (HPTLC) technique or also the Thin-Layer Chromatography (TLC) method for analyzing saponins (in fact, HPTLC is an enhanced TLC).

You can find out useful information about this issue through the four links below:

http://www.apjtb.com/zz/2012s3/45.pdf

http://www.sciencedirect.com/science/article/pii/S0963996914004487

http://www.diva-portal.org/smash/get/diva2:610888/FULLTEXT01.pdf

http://www.researchgate.net/publication/11169375_Chromatographic_determination_of_plant_saponins

Sincerely

Article Chromatographic determination of plant saponins

HPLC is the best method for saponin but you can also adopt the following steps

The brief steps are:

Conduct preliminary analysis using laboratory chemical test methods. Then run TLC to know the type of fractions. After that Column Chromatography with HPLC, after isolated pure fraction run FT-IR, LC-MS, HNMR, CNMR for structure elucidation. You can read my papers too.