I want to use CAS9 systems to knock in expression cassette into CHO cells to make stable cell lnes. how do I choose the hot spot for knocking in?

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Subin R. C. K. Rajendran

You should refer to the safe harbor loci in the human genome.to which, the transgene should be preferable targeted.

Article Functional genomics, proteomics, and regulatory DNA analysis...

Article Safe harbours for the integration of new DNA in the human genome

Beera Shankar Anand

Xiang-Bin Wang I learnt that safe harbor landing site for CHO cells is H11 (Hipp11) locus. as such there are no ready kits available for CHO cells as it was there for Human and Mouse from origene.

this link might help

Article A system for site-specific integration of transgenes in mamm...