Does anyone know how to design primers for promoters?

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Is there any database of free acces to obtain raw data of different materials using x ray diffraction?

I´m interested in to obtain raw data of x ray diffraction of different materials from open acces database for my students, in order to train it in retvield refinement using Match software or...

28 August 2023 9,953 2 View

Can someone share with us the ISO 2631-5: 2018 standard?

we need the ISO 2631-5: 2018 standard, to finalise our comparative analysis with the data obtained from measurements.

10 June 2023 3,676 0 View

Artificial Intelligence, an enabler for Digital Equity and Inclusion?

I am interested in finding out if anybody is working on the subject of "Artificial Intelligence as an Enabler of Digital Equity and Inclusion". I will be very grateful if you share your...

07 June 2023 8,855 4 View

¿ Como poder identificar a estudiantes universitarios en el entorno académico, con trastornos en su salud mental? existe algún estrategia pedagógica?

La salud mental se ve afectada por muchas situaciones de la vida cotidiana, pero lograr identificar un desequilibrio mental considero que, no resulta fácil.

30 March 2023 7,663 0 View

Researchers interested in OBD-2 automotive data processing?

I invite national and foreign researchers to collaborate in the analysis, diagnosis, prediction and ideas that arise for the processing of data obtained through the OBD-2 connector in different...

11 February 2023 4,620 0 View

How can I calculate a sample size for a matched 1:1 case-control study to determine risk factors for SAM (severe acute malnutrition) ?

I am trying to design a study to determine risk factors for Severe Acute Malnutrition. The study would be in a hospital setting where cases would be infants

04 September 2022 2,598 1 View

Could Rhodamine 6G signal not be detected in liver?

We made a pilot study with N=2 mice per group to test biodistribution of rhodamine 6G-loaded silica nanoparticles functionalized with PEG, at 1.5h, 6h and 24h. We used GFP detector in IVIS...

27 April 2022 8,846 2 View

What kind of verification for the effiency of cysteine thiol residues labeling do you use in your experience?

I have been working on the labeling of cysteine thiol residues using a differential alkylation technique (NEM and Biotin Maleimide), during the whole process before performing the trypsin...

04 April 2022 7,232 1 View

Best ways to spike soil with PFAS (PFOA, PFOS, and others)?

I would like to do some experiments on soil treatment of PFAS. I have some ideas on spiking the soil, but I would appreciate hearing ideas, especially from people who have experience doing...

24 January 2022 4,090 0 View

Setting mass yield constraints in RGibbs reactor (Aspen Plus)?

In Aspen Plus I have the following problem (see attached figure): I have a RGibbs block which calculates a distribution for a predifined set of products. However, I would like to go beyond this...

30 November 2021 5,540 1 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Research Methodology - Impact of Corporate Reputation on Stakeholders Behaviors?

Please can anyone support with the survey questions based on RQ measures and propose how to do it in FMCG industry and include as well the role of brand equity Thanks

06 August 2024 949 0 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Should I remove an item from a scale to raise Cronbach's alpha and McDonald's omega or is it better to leave it if they are both over .7 already?

Hello! I have this scale which had 10 items initially. I had to remove items 8 and 10 because they correlated negatively with the scale, and then I removed item 9 because Cronbach's alpha and...

01 August 2024 4,606 7 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View