Has anyone had issues staining AAV injected tissue with immunofluorescence?

I am injecting the hippocampus with an AAV8-GLT1 viral vector. I noticed when I am staining with immunofluorescence the secondary antibodies (Ms 598) is sticking to AAV virally injected tissue...

19 March 2020 5,961 5 View

Can anybody help me with the protocol on how to detect protease activity in a gel containing Casein substrate?

I have just cloned and got a soluble expression(50%) of a thermophilic serine protease and did the heat treatment for purification at 70 and 80 degree with over 90% purity, and also have a...

15 March 2019 9,330 3 View

What are best purification buffers and their conc. that I can use to purify a 6xHis- tagged recombinant serine protease using Ni-NTA resin ?

I have just cloned and expressed a thermophilic alkaline serine protease in E.coli BL21(D3). The protease was expressed as a soluble protein (about 60%) with about 30kDa Mol. wt. (confirmed by...

27 December 2018 1,777 7 View

Can i cytospin cells that are already fixed and stained for IF onto glass slides for confocal imaging?

I plan to isolate breast cancer cells from patient blood samples and would like to fix and stain them in-situ, within the Eppendorf, and then spin them onto a (poly-l-lysine-coated) glass slide...

14 August 2018 3,354 3 View

Can MOLT-4 cell be stimulated with anti-CD3/anti-CD28?

I am looking for a cell line other than Jurkat cells that can signal through the TCR. Would this be possible with MOLT-4 cells or do you know of other cells lines that I could use?

07 June 2018 651 3 View

I'm running a comeptitive ELISA and when i add my 0.15M HF stop solution (with 0.1MEDTA) the net OD decreases and it should increase. help!?

I'm running a comeptitive ELISA and when i add my 0.15M HF stop solution (with 0.1MEDTA) the net OD decreases and it should increase. help!?

31 May 2018 9,776 2 View

What is best temperature for the incubation of thermophilic bacteria on liquid broth for the purpose of DNA extraction?

I incubated my organism (thermophillic bacteria) on LB broth at 37 and 40 degrees centigrade for the purpose of extracting DNA from the organism but did not show any growth after 22hrs of...

05 October 2017 601 11 View

Are there any publically available DNA database for recA gene sequences to use in metagenomic analysis?

14 August 2017 1,969 2 View

Collaborative research on investigating changes of cannabis flower throughout curing?

21 May 2017 4,620 2 View

Is anyone aware of smart phone apps or programs to measure the raw acceleration signal from the phone?

Looking for a way to measure, record, and export raw acceleration from the smart phone's internal accelerometer.

30 November 2016 7,438 6 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Can we recognize signs of the "volatility clustering" property of a time series in its time domain plot?

My purpose is to recognize the volatility clustering property without statistical tests such as The Lagrange Multiplier (LM) test.

24 February 2021 4,150 3 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

How to run repeated measures in mvabund?

I have a dataset of fish species abundances collected from 6 different habitats over the course of 30 years. Fish were collected multiple times per year and environmental variables were recorded...

21 February 2021 3,838 1 View

Is there a method for evaluating nanoparticle adhesion to epithelial tissue in vitro?

I want to measure how well nanoparticles can transfect cells based on NP adhesion properties. In the literature I find people do it in animal studies, and epithelial adhesion helps in transfection...

21 February 2021 7,791 3 View

Any Homemade Organic Synthesis Practices?

Hi everyone, I am a teacher of Heterocycles Synthesis, Is there a home practice that you recommend for undergraduate students? A simulator would also be useful Due to the pandemic, students...

18 February 2021 2,769 4 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View