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Questions related to Biochemistry
Dear ORCA User, I am trying to find out the transition state of a reaction using NEB-CI but not able to converge the transition state. The example given in the ORCA input library works fine. Even...
03 July 2021 2,264 0 View
Does anyone know of a functional Cre - fluorescent protein fusion, other than GFP? I have found one example of a cerulean fluorescent protein - Cre fusion vector in addgene but no data to show...
28 June 2021 439 0 View
Experiment: Weight of sample dried : 25g of leaves Weight after Soxhlet extraction: 100ML The crude extracted were not dissolved because it is aqueous volume Amount used in FC assay: 500µL of...
17 June 2021 545 0 View
The wetting agent is used in the textile industry during the pretreatment process of Dyeing in order to improve the absorbancy of chemicals & dyestuff.
16 June 2021 4,601 2 View
Does anyone have any experience in transformation of EcN (E. coli Nissle 1917), I have been trying different protocols: heat shock, electroporation and conjugation and until now, not a single...
04 June 2021 2,080 0 View
Hello, everyone. In SPR or BLI, I wonder if apparent difference of KD (binding affinity) requires comparison. I heard at least 10-fold comparison of KD means apparent difference. Then, less...
01 June 2021 664 4 View
Does anyone have access to a Pierce (ThermoFisher) fast semi-dry blotter 88217 manual that they're willing to share. Thanks for your help!
27 May 2021 2,638 0 View
The SAR data shows decorrelation for highly vegetated terrains. Has any of the latest software overcome this shortcoming?
27 May 2021 1,517 6 View
Bellow I given a Chemical Composition of 1060 grade aluminum tube. What is the effect if I increase or decrease the Silicon in an aluminum tube?
25 May 2021 2,584 1 View
Hello, we are using EDC for activation of COOH groups and after conjugation with amine group (reaction is 12h in fridge and dark) we want to block rest of COOH. In Hermanson is used EDC and...
10 May 2021 4,132 3 View
I am actually doing protein precipitation using AS as first step of purification of an alpha-amylase. I grew my Bacillus strain an optimized medium for 24h then I spinned-down at 4500 rpm for...
05 May 2021 2,825 10 View
Could anyone suggest to me the eutectics of these carboxylic acid buffers and the relevant articles related to the eutectic points of buffer solutions?
19 April 2021 9,238 0 View
Copper and Cobalt can improve osteogenic differentiation of mesenchymal cells, but can it be somehow connected with hyaluronic acid?
17 April 2021 7,976 4 View
I am preparing approx 450nm particles, but they have a high dispersity in size (15%). How can i use a centrifuge to remove larger particles (approx 600nm) and get the dispersity down to
16 April 2021 2,711 2 View
I would like to measure the inflammatory response triggered by human stem-cell derived neurons with/without focal delivery of anti-inflammatory molecule. To do it, I'd like to establish a...
12 April 2021 7,040 1 View
Dear friends of RG. I have many protein sequences (of a single unstructured domain) and I want to perform an analysis for the prediction of disorder at their specific residues in order to...
06 April 2021 6,290 3 View
Agilent genotypes for these strains list a cryptic Amy gene in the F' of some of their cloning strains; tech tells me its an alpha-amylase gene that mat have been a "side-product" of the plasmids...
16 March 2021 9,254 0 View
The combined oral contraceptive pill is often just called "the pill". It contains artificial versions of female hormones oestrogen and progesterone, which are produced naturally in the ovaries. If...
04 March 2021 454 6 View
Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...
24 February 2021 8,901 5 View
I obtained a full-length coding sequence of certain alpha-amylase. I also determined the signal peptide for the CDS. In the next step, I would like to perform protein structure prediction with...
09 February 2021 6,373 2 View
I am using ( Scientific Simethicone Wrist Action® Shaker Model 85-AA 115V) as a reference for testing my accelerometer. So how I can calculate the amplitude of acceleration (m/s2 or g) at each...
01 February 2021 9,680 0 View
We sourced sodium hyaluronate that I need to convert to hyaluronic acid for drug delivery purposes. How can I do that? Would an ion-exchange resin do the job? Do you know of an established...
20 January 2021 8,820 0 View
I always find that after using Ag/AgCl electrode in a neutral buffer, the potential after calibration changes consistently? So I'm wondering what could be the reasons for this?
08 January 2021 1,211 0 View
Am in Peptide / Protein Research, may i know what useful analytical information we are getting from 13C & 1H NMR spectroscopy analysis? Somebody getting NMR for smaller proteins, any useful...
06 January 2021 1,567 7 View