This group is designed for the exchange of thoughts on Biochemistry | Contact experts in Biochemistry to get answers
1,981 views 2,279 posts
Questions related to Biochemistry
Hello everyone, My question is the next : Why the DHB matrix is a reducing agent while sDHB matrix is not a reducing agent? In this article it is written that sDHB is a non reducing agent Fast...
10 October 2017 6,631 4 View
Some old papers (from the 70s) claim, that the substitution at position 7 is rare in the nature https://www.ncbi.nlm.nih.gov/pubmed/486500https://www.ncbi.nlm.nih.gov/pubmed/4640371 However, that...
10 October 2017 7,942 2 View
D-ribose has demonstrated significant enhancing abilities in replenishing deficient cellular energy levels following myocardial ischemia, does the metabolic pathway differ from that of D-glucose?...
29 September 2017 8,270 2 View
I have some FAD that has been stored at room temperature for a bit over a month and am wondering if it is still good or if it has degraded somehow.
29 September 2017 8,781 1 View
Does anyone know what the biological function of hydroxypyruvate isomerase (Hyi) is in animal cells? I know that it catalyses the chemical reaction of hydroxypyruvate to 2-hydroxy-3-oxopropanoate,...
20 September 2017 6,765 3 View
My protein has no particular tertiary structure and has a pI of 5. It is overexpressed in Rossetta cell line with His tag. After Ni-NTA column, there is lots of DNA in my sample. I tried DEAE...
11 September 2017 4,333 9 View
I have some glucose oxidase that has been stored for about a year in a freezer. Is it still good or do I need to replace it?
09 September 2017 2,555 2 View
09 September 2017 5,005 1 View
Hi, I have a very general question. Since PBS is a isotonic solution, I was wondering if 2X or 0.5X PBS could be considered as hyper or hypo osmotic conditions respectively? I know that typically...
09 September 2017 2,250 3 View
09 September 2017 2,795 2 View
Hi, I was wondering if any one could explain me the relationship between mitochondrial membrane potential and superoxide production? In order to maintain its functionality, mitochondria need to...
09 September 2017 8,797 3 View
I have a protein that doesn't have a Vmax --- this means that the activity keeps increasing no matter how much substrate I add. I've seen people report Kcat/Km in this case, even without...
08 August 2017 7,897 12 View
Hi, I'm doing a MD study about DBD of Glucocorticoid receptor. Why do it have two Zn motifs ( protein chains)? Both have same function or different functions? What is the importance of having two...
08 August 2017 9,423 4 View
I am going to do alcian blue staining for cartilage and the final step is to use trypsin in saturated sodium borate solution. I know sodium borate solution avoids to lose calcium during the...
26 July 2017 8,188 3 View
Does anyone known of any antibodies I can use to tag CRF1 and CRF2 receptors?
24 July 2017 8,954 1 View
Am getting a chaperone at around 63 kD . I have my construct in pETM41. Am purifying with amylose resin and getting a chaperone after affinity. Will HIC after first step clean my protein?
22 July 2017 893 4 View
How can you determine polarity of solvent mixture for using it in tlc
20 July 2017 2,115 3 View
When trying to determine if a molecule is generally unstable or not, what do you look for? Also, is it possible to determine exactly how it will degrade? For example, if I have an aqueous solution...
15 July 2017 5,919 3 View
protein, protein dimerization, buffers role, salts role in proteins precipitation, proteomics, protein crystallography
14 July 2017 9,674 6 View
Hi, I need to know the sequence of pGro7 to check the transformation but I can't get the sequence on the internet. Neither can I request it to the distributor due to a collegue lent me a few...
14 July 2017 4,296 4 View
I have a small M.W. drug that is known highly bound to plasma protein, but I am not clear what kind of methods are able to accurately determine the Kd of this type of binding.
13 July 2017 7,087 3 View
I need to compare isolated and over expressed alkaline protease thermo stability with already industrially applied alkaline protease.What is the best software for that thermostability calculation...
12 July 2017 7,527 3 View
I am to propargylate cyclodextrin using LiH. However, it seems that LiH is very reactive to moisture, making its handling very difficult to do. Can I get some tips/pointers on handling such...
08 July 2017 5,049 2 View
I want to lyze exosomes or extracellular vesicles in a moderate solution and the nucleic acids in the lysate can be used for PCR or isothermal amplification methods without any extraction or...
07 July 2017 167 0 View