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Questions related to Biochemistry
I have been considering the number of MGB probes I can use in my multiplex real-time PCR. The conventional advice suggests using up to two MGB probes, with the third probe being 'normal' - without...
13 November 2023 401 2 View
I have to perform an amplicon sequencing analysis (metabarcoding) with the plant primer trnL intron g and h. For the sequencing I need to add 5´ tags to the primers. There is a methodology to add it?
09 November 2023 3,750 0 View
Greetings everyone, I am trying to dock a planar ligand with a receptor molecule using Autodock tool. I want to freeze the torsions of the ligand. So, I changed the number of active torsions to...
08 November 2023 7,604 3 View
Surangin is a coumarin. Wanted to know if it is available in Indian market as a standard/marker?
26 October 2023 8,456 0 View
Kindly explain
20 October 2023 9,442 4 View
Would it be possible for someone to kindly assist me in calculating the LD50 value using JMP 17 software? Your guidance and support on this matter would be greatly appreciated.
20 October 2023 9,340 0 View
I'm purifying the phi29 DNApolymerase, but the protein activity is always low and it's accompanied by very serious nucleic acid contamination. I once purified a batch of highly active protein, but...
19 October 2023 835 1 View
i am working on the determination of the synergism activity of the herbal and allopathic drug. firstly i tried to find out the zone of inhibition of individual drug having lower (25, 50, 100 ppm),...
13 October 2023 149 3 View
What is the effect of the Ashwagandha plant on pregnancy and fetuses?
11 October 2023 2,769 3 View
Trying to write a bachelor thesis.....
09 October 2023 4,154 0 View
In their website they mentioned it's IF is 5.8. But in the JIF2022 report, I did not find. Is it because of its inclusion in the Emerging Source Citation Index? and because of not included in the...
08 October 2023 1,028 1 View
What is difference between double beam UV spectrophotometer and UV-Vis double beam spectrophotometer?
04 October 2023 5,642 4 View
Now I have Sodium hyaluronate solution from 0.1%to 1%. What range of RPM would be feasible to make sodium hyaluronate hydrogel by using a high speed centrifuge?
27 September 2023 6,122 0 View
Now I have sodium hyaluronate(SH) powder which is 7kda to 12kda and 75kda to 90kda. What concentration will be suitable to make SH solution to make hydrogel with good mechanical performance?
27 September 2023 7,665 0 View
Now I have sodium hyaluronate powder which is 75kda to kda, what solvent should I choose to make a 1% sodium hyaluronate solution. Is PBS solution or water suitable in this case?
25 September 2023 2,714 1 View
Hi all, I am wondering how I can combine the trajectory in GROMACS. I used the coding 'gmx trjcat -s xx.tpr -f xx-1.xtc xx-2.xtc xx-3.xtc -o xxx-combined.xtc' and 'gmx trjcat -s xx.tpr -f...
17 September 2023 2,499 0 View
Hi please can anyone guide me on how to retrieve clonal complex (CC) from MLST sequence of seven house keeping genes of E. Coli, and also to do spartial distribution of the CC. Also, due to...
15 September 2023 5,220 1 View
dose titration , dose escalation
14 September 2023 1,229 1 View
My nanopore sequencing run has generated some ''unclassified reads''. Can anyone explain what causes them to be unclassified and how to avoid them in future?
14 September 2023 1,342 3 View
I wish to study dye degradation using these nanoparticles but I am unable to find adequate research papers on biosynthesis of iron-selenide nanoparticles.
09 September 2023 9,948 4 View
There are different methods to determine LD50 of chemicals and xenobiotics, these include Lorke's method, up and down method, Karber's method, Reed and Muench etc. But which of those is relatively...
02 September 2023 2,671 0 View
Does anybody have a reliable method of being able to measure increased microbial biomass during a ready biodegradation test (e.g. OECD 301F) between the beginning and the end of the test (or even...
29 August 2023 7,512 6 View
Why does water have a high boiling point and surface tension and type of bond is responsible for water molecules sticking to other water molecules?
28 August 2023 9,638 0 View
I have been through the various paper where mostly the works of Chan has been cited. Does anyone have the laboratory protocols in the determination of very labile, moderately labile, less labile...
24 August 2023 1,062 0 View