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Questions related to Biochemistry
Dear colleagues, I am currently conducting research on the criteria used to select pancreatic donors for islet cell transplantation and would greatly appreciate your insights on the following...
08 February 2025 5,209 0 View
I recently received a lyophilized enzyme (5U Glutamate oxidase) from Sigma-Aldrich, when I was about to prepare some aliquots, I noticed that some of the powder is stuck to the cap. I want to...
06 February 2025 7,638 2 View
PK is copolymer of CO, ethylene and propylene. according to information from Internet, melting point of Polymers that alternately copolymerize carbon monoxide and ethylene is 260°C. melting points...
05 February 2025 2,130 1 View
I wanted a JCPDS No:44-141 reference raw file to match with alpha MnO2 XRD. Can anybody share with me here.
04 February 2025 5,641 4 View
I am doing a GST-pulldown. For this, I am coupling GST magnetic agarose beads to my GST fusion proteins; after that I incubate this mixture with my cell lysates. I've made a mistake and started to...
01 February 2025 8,187 3 View
After processing testicular tissue and isolating haploid germ cells, I fix them in 70% ethanol and store at 4 degree C. I usually perform flow cytometry cell cycle analysis 2-3 months after sample...
30 January 2025 7,113 4 View
I am currently trying to optimise a method for Mitochondrial complex confirmation in Blue Native Page. I have transferred my gel to PVDF and incubated with OXPHOS antibody cocktail. I struggled to...
21 January 2025 7,796 0 View
I have performed Brine shrimp lethality test (Cytotoxic) on a plant extract. Due to limitation of laboratory based resources I could not perform any other cytotoxic/anti-proliferative test. I have...
20 January 2025 5,774 1 View
Hey all, is someone using Drosophila files carrying a mutation in the fmr1 gene in particular the Delta50. All my stock are heterozygotes for the mutation being Tb... what do researcher for...
16 January 2025 4,267 0 View
Seeking Expert Feedback on My Review Paper! Dear colleagues, I am currently finalizing a review paper titled "Novel approaches for articular cartilage damage and regenerative medicine”, I am...
12 December 2024 9,875 5 View
Hi everyone, I'm currently using AI to predict binding interfaces in a protein complex. I used models from AlphaFold2, Rosetta2 and ChaiDiscovery. I analyzed the models with Chimera using the...
12 December 2024 701 1 View
Hi guys, I am looking at fluorinated USC compounds and degradation compounds by LCMS with C18 column using ammonium acetate in H2O (MP A) and MeOH (MP B). I am running some samples in scan mode...
09 December 2024 933 0 View
I have prepared an oil in water nano emulsion and would like to visualise it for confocal microscopy, however i dont have any fluorescent compounds in my emulsion. I know that nile red is a...
05 December 2024 6,328 1 View
In HPLC-UV analysis of propolis extract, what is the best analysis conditions and what is the wave length detection is-it 290 single channel?
05 December 2024 3,656 3 View
I am using the High Capacity cDNA Reverse Transcription Kit from Thermo Fisher and the GeneAmp® PCR System 9700 thermocycler to synthesise cDNA. The method specifies the following protocol: Step...
26 November 2024 4,565 4 View
Hi, Ive recently conducted a thermal shift assay on my protein sample. My protein is in 10mM phosphate buffer with 0.1 % Triton X-100. for the record i use the default setting on Biorad qpcr...
25 November 2024 5,850 5 View
As per my knowledge, all the Journal guidelines mention Author names and affiliations for manuscript submission. Then how can an independent researcher with enough research credentials publish a...
23 November 2024 9,766 4 View
Just need to know if yes which company
12 November 2024 9,294 2 View
do you have a job positions about conventional wheat breeding programs.
08 November 2024 1,143 2 View
During MT measurements, the bifurcation of ZFC FC is there till 350 K with a slight ZFC broad peak at 300 K at 100 Oe applied field. Also the moment of both ZFC-FC at low temperature is taking...
07 November 2024 7,986 1 View
Dear all, I have predicted the AsO4 vibrations for lapeyreite (monoclinic, C2/m) with Bilbao server, but I would like to predict also the lattice vibrations, in order to compare the experimental...
07 November 2024 8,630 0 View
RNA isolation from the plant leaf samples.
07 November 2024 9,162 1 View
I'm studying elephant-human interaction, focusing on Sri Lanka at the moment. I am very familir with the work in South AFrica on using contraception to help "control" elephants numbers, and I am...
01 November 2024 5,983 5 View
What is female sexuality? Does it include pregnancy, childbirth and menstruation?
01 October 2024 6,157 1 View