Could you give some suggestions on N-TAILS analysis?

Hi I am planning to do protein N terminal amine isotope labelling analysis (N-TAILS) to find the substrate proteome of my protease of interest. For this I have the WT and the knock down line for...

09 October 2019 6,576 3 View

Is fixation of polyacrylamide necessary for autoradiography?

Hi, I am doing a pulse chase experiment and looking for 35-S methionine/cysteine incorporation in my protein of interest. Following gel run, I directly dry the gel (10% SDS-PAGE, 1.5mm thick,...

06 July 2019 1,175 8 View

How could a heterologous expression affect the conformation of a non-cytosolic protein?

Hi, I am working with a protein that is typically secreted from the parasitic cells that I study. I am trying to express and purify it from HEK cells. Despite of using the mammalian signal...

03 April 2019 7,395 6 View

Has anyone used cellstripper for Leishmania/Tryapsnosoma?

Hi, I am doing starvation assays with Leishmania parasites for which I need to culture parasites in PBS for about 6 hrs. Typically I find that without serum supplement these cells become very...

31 December 2017 2,097 3 View

How to make urea-sds-page gel?

Hi, I want to make a 10% SDS-PAGE gel in which both the resolving and the stacking would contain 6M urea. How would I prepare them? I have already prepared my samples in buffer containing...

09 October 2017 2,442 4 View

How to make urea-sds-page gel?

Hi, I want to make a 10% SDS-PAGE gel in which both the resolving and the stacking would contain 6M urea. How would I prepare them? I have already prepared my samples in buffer containing Tris-Cl,...

09 October 2017 8,003 2 View

How to make urea-sds-page gel?

Hi, I want to make a 10% SDS-PAGE gel that contains 6M urea. Both the resolving and stacking buffers should have urea. Could anybody please suggest how to do this? I prepared my samples in buffer...

09 October 2017 402 2 View

How is superoxide production related to mitochondrial membrane potential?

Hi, I was wondering if any one could explain me the relationship between mitochondrial membrane potential and superoxide production? In order to maintain its functionality, mitochondria need to...

08 September 2017 8,709 3 View

How to create hyper/hypo osmotic conditions?

Hi, I have a very general question. Since PBS is a isotonic solution, I was wondering if 2X or 0.5X PBS could be considered as hyper or hypo osmotic conditions respectively? I know that typically...

08 September 2017 2,156 3 View

Can I use flow cytometry for measuring free cytosolic Calcium conccentration?

Hi, I work with Leishmania parasites and would like to measure the free cytosolic Calcium concentration in them. For this I plan to use Fluo 4-AM dye from Thermo. As I see mostly people use some...

06 July 2017 2,566 5 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

How do I determine the degree of polymerization of PEG 400 based on NMR integration?

Hello, I am working on the photocatalytic degradation of PEG 400. I would like to know if it's possible to determine the degree on polymerization based on NMR integration, or by the MW of the...

02 March 2021 6,186 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View