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Questions related to Biochemistry
I am using MG-RAST to analyse metagenome datasets and have used the Subsystems, KO, NOG, and COG databases for annotation of the functional genes. However, I don't know enough about the different...
06 June 2019 4,970 3 View
We need to collect the serum sample for the analysis of liver function.
05 June 2019 8,105 3 View
I want to stain the fibrin network of a hydrogel constituted of fibrin for confocal microscopy. However, I did not find dye that would directly stain for fibrin. Is there any? If not, does anyone...
27 May 2019 2,821 2 View
please, I need to find 2 articles, 1. G.L EllmanA colorimetric method for determining low concentrations of mercaptans Arch. Biochem. Biophys., 74 (1958), pp. 443-450 2. G.L Ellman, D Courtney, V...
05 May 2019 7,414 5 View
Any details for the timing/specifics of processing the blood would also be appreciated; eg can the plasma be stored in a freezer for some time?
07 April 2019 5,706 0 View
Hi, I'm trying to make my own borate buffer as per the specification in the picture attached. Concentration of 0.4 N, and a pH value of 10.2. I found a lot of different recipe online and I came...
04 April 2019 5,426 4 View
Hi, I am working with a protein that is typically secreted from the parasitic cells that I study. I am trying to express and purify it from HEK cells. Despite of using the mammalian signal...
04 April 2019 7,484 6 View
The identification of serum tuftsin by reverse-phase high-performance liquid chromatography and mass spectrometry. Naim JO, Desiderio DM, Trimble J, Hinshaw JR. Anal Biochem. 1987 Jul;164(1):221-6.
22 March 2019 9,929 1 View
03 March 2019 619 2 View
Hi, I'm trying to figure out my buffers for a membrane protein and was looking at the Dynabeads CoIP kit. We already have the Dynabeads and I was wondering if the composition of the buffers in...
03 March 2019 5,883 4 View
Dear Colleagues, I need to know the accurate definition of these terms in biochemistry: Zwitterion, Isoelectric point, and Sensitizer? Kind regards, Homayoon.
03 March 2019 346 3 View
I am interested in purifying proteins with 1 or 2 disulphide bonds, and have been using enterokinase to cleave off N-terminal tags but we have had issues with poor cleavage and want to try TEV...
11 February 2019 2,214 3 View
I have 5 M Nacl , 2M Buffer and 30% Glycerol How to prepare 500 ml of solution containing 400 mM Nacl and 50 mM of buffer. Thanks in advance.
06 February 2019 7,745 2 View
1.Activity of Hydrolytic Enzymes in Various Regions of Normal Human Brain Tissue M. Prabha • V. Ravi • N. Ramachandra Swamy Ind J Clin Biochem (July-Sept 2013) 28(3):283–291 DOI...
02 February 2019 3,279 4 View
I would like to know whether the Master degree is enough to apply a jobs in journal editorial board. If possible How can I apply such a post. Some one kindly help with a link, where such available...
02 February 2019 1,849 3 View
I am trying to fix the nucleosomes by formaldehyde. The idea is to make the nucleosomes stable at high salt concentration and temperature by crosslinking DNA with Histone octamer. We have...
02 February 2019 7,262 4 View
Actually I need a more clear explanation than does the Google.
02 February 2019 2,793 7 View
Hello amal, Can you please help me with the protocol for detection of lipase activity using pnpp.?
21 January 2019 2,245 4 View
In researches biochemistry
12 January 2019 8,922 4 View
Dear Scientific Community What are the Chemicals Companies that can produce highly purified products (Antioxidants and Toxins) with adequate quantities and suitable prices, any...
01 January 2019 9,365 5 View
There are many research articles exploring alpha glucosidase, salivary and pancreatic alpha amylase inhibition as a therapeutic target for reducing postprandial hyperglycemia (PPHG) in type 2...
01 January 2019 1,633 4 View
I want in fields other
01 January 2019 6,829 0 View
α -tocopherol is the major vitamin E present in green plant tissues, more specifically in the membranes of chloroplasts and thylakoids. I performed many protocols to detect and quantify α...
14 December 2018 7,792 18 View
What would be a good conference/symposium/congress that would be suitable for the research of new methods where in-vitro experiments of Layer-by-Layer covering of bacteria is studied? If anyone...
13 December 2018 3,532 3 View