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Questions related to Biochemistry
I am trying to assess the detoxification ability of a bacterium encapsulated in different materials. My current approach of measuring detoxification involves measuring the concentration of the...
15 June 2022 7,120 0 View
Hi everyone, For my thesis, I am investigating the effects of MDA and 4-HNE in seminal plasma in male patients undergoing IVF, and thus determining if certain levels of MDA and 4-HNE effect IVF...
13 June 2022 4,640 0 View
We are looking to derive heart rate and respiratory rate from minute wrist movements in UNCONSCIOUS (mostly motionless) patients, in the hope of eventually developing a wearable to prevent opioid...
08 June 2022 7,908 6 View
Hello everyone. I am Tran and I need your help. I am performing a research of propagation in chayote. Brief work: 1) sterilization of shoot collected from mature plant by 0.05% HgCl2; 2) Creation...
02 June 2022 6,648 0 View
I have to prepare 0.5 M sodium tartrate buffer (pH 4.2) for ligning peroxidase test anyone can please help to share the composition of the same?
30 May 2022 7,678 0 View
Hi, every one, if I want to prepare a tumor cell line expressing OVA to carried out antigen presenting experiment. which fragment I should chose? The full length protein or the fragment of...
27 May 2022 7,878 0 View
Monochloramine (MCA) is used to sanitize water and can be used in the production of e.g. starch from wheat flour. Whenever a small concentration of MCA is added in the production stream we find...
21 May 2022 6,530 0 View
For telomere elongation, 2 μL of telomerase extract with various concentrations ranging from 101 to 106 cells was incubated with 1 μL TS primer (100pmol) in 10 μL reaction buffer (50mM Tris-HCl,...
20 April 2022 9,314 2 View
Activation of telomerase enzyme to increase telomere length in humans.
17 January 2022 9,558 4 View
How to increase the solubility of ammonium molybdate tetrahydrate in water... Kindly suggest any other aqueous solutions.
11 January 2022 4,956 3 View
DTT, b-ME and TCEP are commonly used in biochemistry for the reduction of cysteins. Recently, I investiged the effect of the oxidative state of polymer-binding peptides on their binding behaviour....
05 January 2022 9,300 10 View
Dear Experts, How do I write a netcdf file with VMD from a netcdf/dcd trajectory file? It seems that VMD can read a netcdf file but cannot write it, as follows: Info) Using plugin netcdf for...
23 December 2021 1,160 0 View
We are interested to detect somatic mutation in serum/plasma of brain tumor patients, in samples collected after resection and before start of adjuvant therapy. Is it feasible to get the somatic...
22 December 2021 1,934 5 View
As per literature LOD is calculated using equation LOD = k (Sy/S). Where, Sy is standard deviation of blank value, S is the sensitivity of immunosensor, and k (k=3) is the confidence level factor....
16 December 2021 3,180 3 View
The alkyl or aryl based ligands/passivating agents are frequently used for the passivation of metal nanoparticles or colloidal suspension during liquid-phase synthesis. How much should be the...
06 December 2021 5,893 3 View
The result that i obtained from acetylation process showing that the resistant starch value is higher compared to the amylose content. Theoretically, from understanding is that resistant starch...
18 September 2021 8,471 5 View
Hello. I hope someone can help me or give me an idea on how to prepare HEPES buffer with Chelex-100 using the statement I have read on a published journal. Enyzme (500nM) was incubated with...
25 August 2021 6,135 4 View
We have a spectrophotometer (Tecan Infiniti) for a 96-well plate, but I may also have access to a traditional cuvette spectrophotometer.
19 August 2021 1,880 3 View
It is almost two and half a months that I have started callus induction, co-cultivation and Agrobacterium mediated transformation in three different rice varities. Approximately three weeks ago I...
23 July 2021 7,610 3 View
I was wondering whether anyone has experience buying individual reagents instead of the agilent kit for seahorse. I'm looking to run a glycolytic stress test and was concerned that Agilent uses a...
07 July 2021 6,390 3 View
Dear ORCA User, I am trying to find out the transition state of a reaction using NEB-CI but not able to converge the transition state. The example given in the ORCA input library works fine. Even...
03 July 2021 2,206 0 View
Does anyone know of a functional Cre - fluorescent protein fusion, other than GFP? I have found one example of a cerulean fluorescent protein - Cre fusion vector in addgene but no data to show...
28 June 2021 384 0 View
The wetting agent is used in the textile industry during the pretreatment process of Dyeing in order to improve the absorbancy of chemicals & dyestuff.
16 June 2021 4,556 2 View
Does anyone have any experience in transformation of EcN (E. coli Nissle 1917), I have been trying different protocols: heat shock, electroporation and conjugation and until now, not a single...
04 June 2021 2,005 0 View