How much time does it take to measure the IgG content in human blood utilizing current laboratory methods?

What are the current methods to quantify, measure, and detect the content of Immunoglobulin G proteins in human blood? How much time does it take for such tests to complete? How much does it take...

09 October 2019 3,992 4 View

What are the best softwares to simulate Microfluidic Affinity Chromatography process?

I want to simulate my microfluidic design which utilizes affinity chromatography at micro-scale. I have already worked with software such as Comsol Multiphysics. Is there any other software? Which...

09 October 2019 1,907 1 View

How can I buy/order custom micro sized columns for affinity chromatography?

I want to buy chromatography columns for the affinity-based separation of proteins. The size (diameter) of the column needs to be very small (less than 100um) in order to match the design. Are...

09 October 2019 5,519 5 View

How to find out the type and name of materials by using FTIR analysis?

I have utilized FTIR (Fourier Transform Infrared Spectroscopy) to analyze and detect the material of my sample. The possible answers are obtained and I don't know which type of material they are!...

06 July 2019 4,789 16 View

What is the best method to fine-cut a plexiglass (or similar material) cylinder (d=2cm, l=5mm)?

I want the cutting resolution to be less than 0.1 mm and I don't want to damage the sample.

03 April 2019 6,100 3 View

Are there any methods to detect and find the chemical formula of a polymer (carbon, hydrogen, oxygen)?

I have a polymer material and I want to find out what type it is. I want to know its chemical formula. How many Carbons, Hydrogen and Oxygen are there? I already know that Electron Microscopy...

02 March 2019 611 8 View

What are some simple gearbox designs which could multiply speed/rotation by 2?

I want to find a gear mechanism which doubles the output rotation.

06 July 2018 8,560 9 View

Is there any formula to find convection coefficient (h) for a free/natural air inside a tube (internal flow)?

I want to find the natural heat transfer convection coefficient (h) inside the intestines. I just do not know how to calculate it.

05 June 2018 7,151 3 View

What are the main materials for mechanical bistable structures?

In mechanical engineering, a bistable structure is one that has two stable mechanical shapes, particularly where they are stabilized by different curvature axes. (Source: Wikipedia) I want to know...

04 May 2018 705 5 View

Which Standard Cam Motion is suitable for the y-theta diagram below?

I want a rise-dwell-return motion in half a cycle and I don't need the cycle to repeat. Rise: 0 - pi/4 Dwell: pi/4 - 3pi/4 Return: 3pi/4 - pi for Rise & Dwell V0=Vf=0 a0=af=0 It would be great...

04 May 2018 2,629 1 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

Do you kow of any database containing data on retention time, log P and polarizability for a large number of molecules?

Hi, I am a chemistry undergraduate doing my dissertation project on HPLC. In my project, I need to predict the HPLC retention time of 20 aromatic molecules, the calibration set. To do this I will...

24 February 2021 2,602 1 View

Any easy method of analyzing vitamin D in foods by HPLC?

Looking for method of extracting and analyzing vitamin D in foods by HPLC

24 February 2021 7,524 7 View

What HPLC Marker(s) should be used to detect anthocyanins in a sample?

What HPLC Marker(s) or standard is used to detect and quantify anthocyanins such as Delphinidin, Cyanidin in a given sample of plant extract?

23 February 2021 1,832 4 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View