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Questions related to Biochemistry
As per literature LOD is calculated using equation LOD = k (Sy/S). Where, Sy is standard deviation of blank value, S is the sensitivity of immunosensor, and k (k=3) is the confidence level factor....
16 December 2021 3,167 3 View
Hello. I hope someone can help me or give me an idea on how to prepare HEPES buffer with Chelex-100 using the statement I have read on a published journal. Enyzme (500nM) was incubated with...
25 August 2021 6,120 4 View
I was wondering whether anyone has experience buying individual reagents instead of the agilent kit for seahorse. I'm looking to run a glycolytic stress test and was concerned that Agilent uses a...
07 July 2021 6,377 3 View
Dear ORCA User, I am trying to find out the transition state of a reaction using NEB-CI but not able to converge the transition state. The example given in the ORCA input library works fine. Even...
03 July 2021 2,195 0 View
Does anyone have any experience in transformation of EcN (E. coli Nissle 1917), I have been trying different protocols: heat shock, electroporation and conjugation and until now, not a single...
04 June 2021 1,988 0 View
Hello, everyone. In SPR or BLI, I wonder if apparent difference of KD (binding affinity) requires comparison. I heard at least 10-fold comparison of KD means apparent difference. Then, less...
01 June 2021 596 4 View
Does anyone have access to a Pierce (ThermoFisher) fast semi-dry blotter 88217 manual that they're willing to share. Thanks for your help!
27 May 2021 2,577 0 View
Hello, we are using EDC for activation of COOH groups and after conjugation with amine group (reaction is 12h in fridge and dark) we want to block rest of COOH. In Hermanson is used EDC and...
10 May 2021 4,042 3 View
Could anyone suggest to me the eutectics of these carboxylic acid buffers and the relevant articles related to the eutectic points of buffer solutions?
19 April 2021 9,151 0 View
Copper and Cobalt can improve osteogenic differentiation of mesenchymal cells, but can it be somehow connected with hyaluronic acid?
17 April 2021 7,870 4 View
Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...
24 February 2021 8,624 5 View
We sourced sodium hyaluronate that I need to convert to hyaluronic acid for drug delivery purposes. How can I do that? Would an ion-exchange resin do the job? Do you know of an established...
20 January 2021 8,714 0 View
I always find that after using Ag/AgCl electrode in a neutral buffer, the potential after calibration changes consistently? So I'm wondering what could be the reasons for this?
08 January 2021 1,084 0 View
Am in Peptide / Protein Research, may i know what useful analytical information we are getting from 13C & 1H NMR spectroscopy analysis? Somebody getting NMR for smaller proteins, any useful...
06 January 2021 1,401 7 View
can you descirbe the unity of W (mMho) in the impedance spectroscopy
04 January 2021 3,304 1 View
Sometimes my western blots have very uniform/even background, other times they're very uneven and patchy like in the attached image. Why is this? I thought my blots were drying out during...
06 December 2020 8,910 5 View
I am currently trying to run solubilised mitochondria extracts from mammalian cells through a gel filtration column in order to separate proteins by size & visualise protein complexes. My...
30 November 2020 1,670 5 View
In the past, I've always used Licor's old blocking buffer that included sodium azide. I would dilute my antibodies in the buffer, stain, re-collect the dilution, and re-use it a few times. The...
29 November 2020 6,009 3 View
I want to reduce the disulfide bridge in my 7kDa protein. My protein has only 1 cysteine at the surface and thus makes a homodimer using a disulfide bridge. I want to reduce the disulfide bridge...
25 November 2020 3,366 5 View
I prepared a 5X of M9 minimal salt and 1000X elemental trace and mixed these 2 solutions to produce carbon-free minimal salt media. Biphenyl was used as a carbon source but was prepped separately...
21 November 2020 6,049 5 View
Camels milk does not coagulated as a cow milks Naturally.
17 November 2020 2,627 8 View
I'm setting up a liquid handling robot and need a simple assay to test it. Ideally I would like to mix 3-4 reagents and have a absorbance/fluorescence readout on a plate reader. It's also possible...
12 November 2020 7,770 3 View
For western blots, I normally stain with 10 mL buffer with 1:1,000 dilution of my specific antibody. Recently, there is an equipment change in my lab and 10 mL isn't enough volume to cover the...
28 October 2020 2,931 7 View
I had submitted the structure but did n't get any result. Can anyone suggest possible solution of that problem? The model structure is obtained from de novo based process.
06 October 2020 4,848 3 View