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Questions related to Biochemistry
I am trying to extract DNA from skin swabs of Salamandra atra but the DNA concentrations I get are very, very low (like < 0,5 ng/µl). I am using the Qiagen QIAamp DNA Micro Kit in combination with...
23 August 2023 2,523 2 View
Hi all! I recently started performing enzymatic treatment on Nannochloropsis oceanica in order to disrupt the cell wall. Throughout the different extracellular components I want to analyze, there...
22 August 2023 6,106 1 View
How many PBMCs can I seeded for 5 days in a 96-well plate in 200 µl RPMI with FBS and PHA?I started with 30,000, and I don't know if it's not enough
14 August 2023 7,389 1 View
I am trying to develop Curcumin bioconjugates by esterifying -OH moieties of Curcumin aromatic rings. By performing UV spectrophotometric analysis of the conjugate (red curve) in a mixture...
02 August 2023 5,131 2 View
We have an oldschool PPMS equipment with ACMS option, and recently got the problem with DC measurements. When starting a measurement it returns: Measuring DC Extraction 8/1/2023 10:54:37...
01 August 2023 4,212 1 View
OK
31 July 2023 6,487 2 View
Please explain an experimental technique to determine the presence of free radicals generates from the rotten edible oils.
26 July 2023 4,064 2 View
I have synthesis by mixing 10 mM AgNo3 and curry leaf extract
25 July 2023 556 1 View
Is there a tool that can output the most correlated/inversely co-related gene combinations from TCGA dataset? I mean WITHOUT AN INPUT GENE. Just give the highest R value combinations from TCGA...
14 July 2023 5,730 0 View
Are there any standards/ limits for respiratory deposition dose like this much microgram of Pm 2.5 is applicable for 1 hr exposure/ if any....I read many papers but I couldn't find it...Kindly...
13 July 2023 8,085 3 View
I would like to do an experiment blocking TNF to reduce neuroinflammation.
10 July 2023 8,113 3 View
The background of my Western Blots is very uneven. I use premade NUPAGE gels and PVDF membrane. For blocking I using intercept blocking buffer for 1 hour at room temperature. Primary antibody is...
03 July 2023 3,470 3 View
Sir, can anyone explain the DSC curve of biomass
26 June 2023 5,917 1 View
Hello all, My goal is to crystallize RNA-bound HIV-I Integrase. But once I mix equimolar amounts of RNA: protein (100uM), it immediately turns cloudy. Buffer of the protein is 20mM HEPES 7.5,...
23 June 2023 7,533 2 View
Aloevera /nanofiber/electrospinning
19 June 2023 6,005 0 View
hi everyone... i am working on bovine collagen protein but i am not getting proper bands in SDS PAGE. please give me some suggestion . thanks.
18 June 2023 6,198 2 View
Looking for Protocol of GRIESS reagent test on brain. many thanks
13 June 2023 7,785 1 View
Currently, i cultured a recombinant protein in E. coli BL21 with a variation in agitation speed. And in my result it showed that an increase in specific growth rate does not correspond to an...
12 June 2023 7,731 4 View
I am testing TCID50 of Influenza virus work seed batch with MDCK cell lines.The experimental process is as follows： 1.Put MDCK cells in 96 well plates at 16000cells/well in DMEM +10% FBS (100ul...
07 June 2023 1,825 1 View
I have decellularized the umbilical cord with detergent based methods, the problem is that I am unable to solubilize it with pepsin and HCl, kindly guide me with the exact protocol of...
02 June 2023 788 2 View
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11 May 2023 3,793 5 View
Hi everyone, I'm performing DFT Calculation / Geometry Optimization of some 5,7-dibromobenzofurane hydrazide derivatives using ORCA 4.2.1 and BY3LP basis set (! B3LYP RIJK def2-TZVP...
09 May 2023 2,052 4 View
Can we use the Independent T-test when standard deviation is zero for one group, or go to non-parametric test? If some values have no variation and others with zero number, the calculated SD is...
05 May 2023 9,392 10 View
Hello, Have you ever extracted nuclei from fixed samples ? When I fix my HepG2 cells with 1% formaldehyde, I noticed that the fixation prevents cell lysis with regular nuclei isolation buffer,...
04 May 2023 517 5 View