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Questions related to Biochemistry
Hi dear researchers Is it right use 1/mmol unit for extinction coefficient (molar absorption coefficient) instead of 1/mM? (I see this in some atticles)
12 December 2018 4,516 4 View
Textbook of Biochemistry.
12 December 2018 8,026 3 View
α -tocopherol is the major vitamin E present in green plant tissues, more specifically in the membranes of chloroplasts and thylakoids. I performed many protocols to detect and quantify α...
12 December 2018 865 19 View
What would be a good conference/symposium/congress that would be suitable for the research of new methods where in-vitro experiments of Layer-by-Layer covering of bacteria is studied? If anyone...
12 December 2018 3,644 4 View
Hello, I am looking for a compound which could selectively quench reactive oxygen species while not quenching carbon centered radicals (for a polymerization reaction). Thanks a lot.
12 December 2018 8,409 8 View
12 December 2018 8,625 7 View
I try to purify a specific recombinantly expressed protein, but I am facing some trouble with my lysed cell solution after centrifugation. Normally I would expect to get a clear solution and a...
14 November 2018 2,980 6 View
Hi, can someone enlightened me on how many grams of Oxalic acid compound and EDTA do i need to mix in a 1 litter dionized water for determination of Ascobic Acid of Plants? i am going to use the...
14 November 2018 638 0 View
Fetuin has some forms like fetuinA or fetuinB. I want to know if there is any similarities or difference between fetuinA and asialofetuin.
11 November 2018 1,294 4 View
Hi all, I would like to know how to remove the excess fluorescent probe from the LUVs prepared after hydration? And how to do it, any procedure to recommend? Thanks.
11 November 2018 6,268 0 View
11 November 2018 1,447 2 View
In estimation of amylose (Juliano, 1971), I used 1N acetic acid but got color developed only in a few samples. And then I used glacial acetic acid (99.5%) in another set of samples and got the...
10 October 2018 8,421 0 View
I made a DTT stock a few days ago and stored it at -20ºC, and when i defrosted it, it seems unable to dissolve in the solution and has a clear white color.
16 September 2018 7,541 7 View
Hi, I'm doing amino acids of animals feed. I used Jajic et al. (2013) 's methods for the acid hydrolysis. Briefly, I will mix 0.5 gram of samples with 7 ml of 6 molars HCl-phenol solution, flush...
09 September 2018 6,542 4 View
09 September 2018 8,720 8 View
Dear all I have performed a simulation for native protein. Now I want to add glucose molecules (to mimic diabetes condition) with 240 mg/dL. How many number of glucose molecules do I need to...
09 September 2018 1,190 4 View
Dear all, Could you suggest any review/paper for a comprehensive list of cholesterol content of plasma membrane in various human cells ? For example, difference in membrane cholesterol content of...
09 September 2018 1,081 2 View
Hello All, I am doing a protein-peptide thermal shift assay in QuantStudio3. I have observed that most of the peptides are giving a significant negative shift in Tm, though some are giving a...
20 August 2018 1,392 4 View
08 August 2018 823 5 View
To all who see this message, I am looking for joining a research team or institution to possibly get paid and do research in Pharmaceutical or Biological Chemistry and in similar fields in any...
08 August 2018 10,020 2 View
If yes, which kinds of radiation?
07 July 2018 2,405 0 View
I have been characterising a new strain of Streptomyces and was wanting to compare it to other similar alkaliphilic strains of Streptomyces. I have read of figures as high as 11.5 but the...
07 July 2018 1,487 8 View
Hi guys. Based on the instructions given by SigmaPlot, in order for me to do the enzyme kinetics analysis, the first step is Entering data into the Data Entry Wizard. However, when I clicked on...
07 July 2018 1,877 3 View
I'd like to quantify the thiol status of cells treated with an alkylating agent. Is Ellman's reagent suitable for use with whole cell lysates? Is there a standard protocol for this assay? Limited...
19 June 2018 9,191 6 View