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Questions related to Biochemistry
I've been trying to synthesize NH2-HA recently, but it failed. Can someone please tell me why and how I can improve it? The synthesis steps were as follows: I dissolved 500mg of HA in ddH2O and...
02 March 2023 2,840 5 View
Elution buffer composition: 50mM Tris-Hcl(pH9.0) + 10mM reduced glutathione + MilliQ water
01 March 2023 8,272 2 View
Is it necessary to have pyruvate in the culture medium when culturing lung squamous carcinoma SK-MES-1 cells? Now the cells appear to be dying and in bad condition after three days of culturing in...
27 February 2023 1,477 4 View
I was reading a few articals for my research on TiO2 used in paint formulation, and I found only few extenders like Calcined kaolin, Hollow spheres, Barium sulphate that could reduce by little the...
24 February 2023 2,514 1 View
This was from a whole cell lysis protocol using 125uL of Y-PER and 2uLof PIC. I agitated the yeast cell pellet twice and then centrifuged the samples. I added 10uL of 2X loading buffer+BME with...
22 February 2023 9,541 1 View
Hello, I bought this antibody to make electrochemical immunosensors, I bought the ab93903 antigen which is recommended. I prepared the antibody to the desired concentration in 10mM PBS pH 7.4 and...
22 February 2023 7,221 3 View
I did check its solubility properties and I found the value of 400 mg/mL = 400 g/L, but despite that, I always observe some crystals in the solution i'm preparing, and it only as a 6 % w/v...
15 February 2023 4,576 1 View
I am in the process of doing my senior research project. I have been referring to various papers to try and find methods to extract and analyze my samples. Most papers say "Mycosporine-like amino...
10 February 2023 6,771 1 View
Hello, I want to isolate the outer membrane protein from a bacterium that I grew under different conditions and display it on sds gel. Sonication device was used in all the articles i found as a...
09 February 2023 9,284 7 View
Suppose if I input "STAT3", is there any database that can show expression changes in STAT3 from different proteomics experiment datasets, in a tabular form (assuming these datasets have been...
07 February 2023 6,601 0 View
Hi everyone, I work with Arabidopsis thaliana. I want to know what is the best strategy to strore plant saplings after the treatment to preserve the quality of RNA (at least for a week). 1. Is it...
04 February 2023 5,531 4 View
hello i am in need for ideas on this question so as i can accomplish my term paper, enlighten please....
01 February 2023 10,022 6 View
I am trying to prepare a Minimal Media with lower and higher pH than usual. I had success with the pH 3 media but the higher pH one is giving me trouble. Right now the media...
11 January 2023 6,594 4 View
In a scenario, where I have about 25 protein sequences of a lytic enzyme (Variants) of bacterial origin, I would like to estimate the mutational distance of each of these sequences and...
26 December 2022 9,756 1 View
The topic of my project is an immunosensor. For that, I dropped casted antibody with nanomaterial on the working electrode, then I kept the electrode at 4 c, but I had to keep that electrode at...
06 December 2022 2,829 4 View
during last 2 year, i have not obtain root from my transgenic poplar plantlets. I previously found the regeneration protocol and obtained regenerated transgenic plants. however, when i tried...
29 November 2022 6,366 0 View
I am carrying out a CRISPR knockout using PX458 plasmid with the following information GROWTH IN BACTERIA Ampicillin, 100 μg/mLBacterial Resistance(s) 37°CGrowth Temperature Stbl3Growth...
07 November 2022 5,526 2 View
Hello for everyone I come to you to help me find a solution to my problem in the GC MSD I did the tune the first time and everything was good after I injected a sample the filament goes out during...
07 November 2022 6,382 4 View
I'm synthesising Cu nanoparticles with EG, PVP, and ascorbic acid. How long the particles will be stable in EG before washing them using a centrifuge?
04 November 2022 1,191 2 View
I am trying to express and purify nanobodies from a nanobody library in E. coli. I am using a pBAD vector to which I added the pelB sequence for periplasmic localization. The nanobody is fused to...
02 November 2022 9,937 3 View
The swissADME tool can calculate iLOGP for a compound. What pH is this measured at?
28 October 2022 5,592 2 View
I am trying to examine the cytotoxicity of an antioxidant agent on undifferentiated SH-SY5Y cells. In my previous try, all of the doses I tried gave results above 100% and all the wells were...
22 October 2022 7,068 3 View
How to estimate the minimum number of subjects that should be enrolled for phase 1 of a clinical trial? The objective of phase 1 is to test for the safety, side effects, dose etc. I know we...
14 October 2022 5,393 5 View
I am trying to perform a biochemical assay which requires me to take readings for reduction in NADH levels at 340 nm. Could anyone kindly suggest what plates should I be using. I currently have...
13 October 2022 837 2 View