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Questions related to Biochemistry
I used unlabeled cells (first image) to set the gate and the second image is my positive control. I am looking for percentage of green cells (FL-1, ''expressing'' cells in my experiment) that are...
11 October 2022 9,092 2 View
Genome sequence data need to be converted into numerical database to perform machine learning algorithm.
11 October 2022 8,466 4 View
I have a catalogue of planktonic organisms that I have identified (mostly to order level) over the years which I would like to identify onto species level and also conduct research on their...
04 October 2022 256 2 View
I have a published phylogenetic tree but no access to the rough data used to generate the tree. I want to compare among clades in terms of morphological characters. How do I include the...
18 September 2022 9,486 1 View
So we do microscale thermophoresis (MST) with labeled protein and labeled DNA. Typically standard titrations are easily performed and there are no problems. However, with some experimental...
16 September 2022 8,841 2 View
Hi, everybody I'm currently producing manganese peroxidase and I need to assay its activity. I'm planning to use the Wariishi's method, it requires to prepare sodium malonate buffer 100mM pH 4.5....
15 September 2022 3,358 0 View
I am using an oncolytic virus for virotherapy and want to determine the MOI of the virus to treat the cancer cell line. I have attenuated the virus in the form of the vaccine, can anyone please...
10 September 2022 9,050 0 View
I am trying to find a way to process raw ballistocardiogram data stemming from an Emft QS bed sensor. Standard software for processing heart rate data from ECG or Photoplethymography such as...
26 August 2022 3,310 0 View
Dear scientists Please inform me which is more convenient centrifuge for chitosan modification? Please suggest the possibility. Thank you
17 August 2022 2,062 0 View
Does anyone know why the peak of purified his tag protein look like this?
03 August 2022 5,209 2 View
I have been trying to do regeneration of rockmelon plant using cotyledonary explant. After a few years of trying, I'm not getting the shoots. I'm able to get up to the callus stage but the callus...
25 July 2022 283 0 View
Tried 0.5-1 mg/ml IAA for rooting but no roots
22 July 2022 2,989 0 View
We are trying to use buffy coat stored in RNALater at -80 Deg for RNA extraction. However, we are unable to get sufficient RNA. Looks like the leucocyte lysis is not happening properly. Any...
21 July 2022 9,174 0 View
I want to know the amount of ferulic acid present in fruit by UV spectrophotometry. Is it possible?
07 July 2022 267 0 View
Dear colleagues, I perform neutrophil isolation with percoll from blood sampling to perform RNA sequencing but when I verify RNA quality (using Agilent RNA 6000 nano), all my RIN are under 5....
04 July 2022 8,022 0 View
I recently made a backup of my data from Geneious prime and now the files are not as a .zip or.geneious etc but as G files. They are not importing into the Geneious Prime software. Does anyone...
22 June 2022 1,789 0 View
I am trying to assess the detoxification ability of a bacterium encapsulated in different materials. My current approach of measuring detoxification involves measuring the concentration of the...
15 June 2022 7,175 0 View
Hi everyone, For my thesis, I am investigating the effects of MDA and 4-HNE in seminal plasma in male patients undergoing IVF, and thus determining if certain levels of MDA and 4-HNE effect IVF...
13 June 2022 4,739 0 View
We are looking to derive heart rate and respiratory rate from minute wrist movements in UNCONSCIOUS (mostly motionless) patients, in the hope of eventually developing a wearable to prevent opioid...
08 June 2022 7,989 6 View
Hi all, I am now conducting my master's research on emerging contaminants in stormwater. The analytes of my interest are: BPA, Triclosan, Glyphosate, and AMPA. I hope to analyze BPA and...
08 June 2022 7,583 0 View
Hello everyone. I am Tran and I need your help. I am performing a research of propagation in chayote. Brief work: 1) sterilization of shoot collected from mature plant by 0.05% HgCl2; 2) Creation...
02 June 2022 6,833 0 View
I have to prepare 0.5 M sodium tartrate buffer (pH 4.2) for ligning peroxidase test anyone can please help to share the composition of the same?
30 May 2022 7,740 0 View
Hi, every one, if I want to prepare a tumor cell line expressing OVA to carried out antigen presenting experiment. which fragment I should chose? The full length protein or the fragment of...
27 May 2022 7,933 0 View
Monochloramine (MCA) is used to sanitize water and can be used in the production of e.g. starch from wheat flour. Whenever a small concentration of MCA is added in the production stream we find...
21 May 2022 6,606 0 View