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Questions related to Biochemistry
Whenever I input my created mesh file in the “Tide Prediction of Heights” on Mike Zero, the longitude and latitude of the start and end points are not automatically detected. And as a result, I...
07 January 2024 2,754 4 View
Hi! Has anyone worked with negative-sense RNA viruses? I need to express recombinant proteins using a vector, so it will be necessary to start with ssRNA(-) to obtain cDNA for selecting the gene...
02 January 2024 9,542 3 View
What are the basic advantages and disadvantages of using both the methods? Which is more helpful?
23 December 2023 9,146 5 View
I am working on the design of the anaerobic baffled reactor for the treatment of dairy wastewater. I need assistance to determine the HRT and OLR for the reactor.
19 December 2023 1,275 3 View
Dear All, Have you tried grafting MA to PP? If so, could you please tell the safety measure and temperature profile to operate the twin screw extruder
06 December 2023 4,630 1 View
I am log-transforming some environmental data for principal components analysis (PCA), in hopes of removing outliers. However, it did not seem successful for many parameters, such as TP, Salinity,...
21 November 2023 7,120 3 View
Hi all, I have been trying to detect several polypeptides by WB. And tricine-sds-page (16.5% or 20%) and glycine-sds page(20%) gel electrophoresis followed by Comassie blue staining showed no...
20 November 2023 7,514 3 View
Hi, To be able to Measure the Total Carbohydrate on my sample, I was planning on using the Total Carbohydrate Assay Kit (Sigma Cat#MAK104). The problem is that it includes a step in which I have...
14 November 2023 8,638 3 View
I have been considering the number of MGB probes I can use in my multiplex real-time PCR. The conventional advice suggests using up to two MGB probes, with the third probe being 'normal' - without...
13 November 2023 492 2 View
I have to perform an amplicon sequencing analysis (metabarcoding) with the plant primer trnL intron g and h. For the sequencing I need to add 5´ tags to the primers. There is a methodology to add it?
09 November 2023 3,827 0 View
Greetings everyone, I am trying to dock a planar ligand with a receptor molecule using Autodock tool. I want to freeze the torsions of the ligand. So, I changed the number of active torsions to...
08 November 2023 7,688 3 View
Surangin is a coumarin. Wanted to know if it is available in Indian market as a standard/marker?
26 October 2023 8,543 0 View
Kindly explain
20 October 2023 9,510 4 View
Would it be possible for someone to kindly assist me in calculating the LD50 value using JMP 17 software? Your guidance and support on this matter would be greatly appreciated.
20 October 2023 9,395 0 View
I'm purifying the phi29 DNApolymerase, but the protein activity is always low and it's accompanied by very serious nucleic acid contamination. I once purified a batch of highly active protein, but...
19 October 2023 895 1 View
i am working on the determination of the synergism activity of the herbal and allopathic drug. firstly i tried to find out the zone of inhibition of individual drug having lower (25, 50, 100 ppm),...
13 October 2023 217 3 View
What is the effect of the Ashwagandha plant on pregnancy and fetuses?
11 October 2023 2,847 3 View
Trying to write a bachelor thesis.....
09 October 2023 4,196 0 View
Looking to write a bachelor thesis on a scientific gap for auto titration, other scientific gap suggestion appreciated.
09 October 2023 6,434 0 View
In their website they mentioned it's IF is 5.8. But in the JIF2022 report, I did not find. Is it because of its inclusion in the Emerging Source Citation Index? and because of not included in the...
08 October 2023 1,094 1 View
What is difference between double beam UV spectrophotometer and UV-Vis double beam spectrophotometer?
04 October 2023 5,695 4 View
Now I have Sodium hyaluronate solution from 0.1%to 1%. What range of RPM would be feasible to make sodium hyaluronate hydrogel by using a high speed centrifuge?
27 September 2023 6,207 0 View
Now I have sodium hyaluronate(SH) powder which is 7kda to 12kda and 75kda to 90kda. What concentration will be suitable to make SH solution to make hydrogel with good mechanical performance?
27 September 2023 7,736 0 View
Now I have sodium hyaluronate powder which is 75kda to kda, what solvent should I choose to make a 1% sodium hyaluronate solution. Is PBS solution or water suitable in this case?
25 September 2023 2,791 1 View