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Questions related to Biochemistry
I am conducting a meta-analysis and in instances where I only have a p-value that is reported as "non-significant", what is the best way to provide some estimate for that p-value. This is so that...
03 October 2020 2,093 9 View
ELECTROMAGNETIC INTERFERENCE SHIELDING WITH TEXTILES-DIFFERENT EMI SHIELDING CONDUCTIVE FILLERS OF METALLIC PARTICLES ON TEXTILE STRUCTURES This is my current research I s implement any novelty...
20 September 2020 4,902 2 View
How difficult is Lysosensor to use in a basic cell culture wet lab? Does anybody happen to have any advice and/or experience with this or similar probes? Thanks in advance!
16 September 2020 8,867 7 View
I must concentrate and purify a lot of dirty lysate and secreted protein samples. I just want to run them on protein gels for western blots. I'm trying to decide the best method of...
11 September 2020 3,080 8 View
I have been searching the literature about this and I have not found an answer. If the atom that accepts a hydride is made more electrophilic, can this speed up the rate of hydride transfer?
30 July 2020 10,072 1 View
I'm trying to perform a BCA assay on some clarified lysate, but the samples appear to become brown, not purple, and the OD562 absorbance is very high. What could cause this? The brown is very...
11 July 2020 7,128 3 View
I am performing a MD simulation using GROMACS 2020.2, with ff-AMBER99sb-ildn & water model-tip3p, for a Zn containing metalloprotein. I have observed that a Zn move away from its position in some...
16 June 2020 4,568 0 View
Various colorimetric assays we use are blue/purple/pink/red but not in green shades. Is there any particular reason for this?
31 May 2020 6,045 1 View
Hello everyone! I have designed a few primers and then a tried optimized the conditions using reference RNA, diferente primer concentrations e annealing temperatures and then calculating the...
26 May 2020 5,860 15 View
Does anyone know if there's a company willing to do all of the following: - Synthesize a custom gene and clone it into a plasmid - Transform E. coli with said gene and develop a protein...
20 May 2020 2,079 9 View
Dear community I have been working on a research related to the milling effect on the physicochemical properties of a whole flour product. Analyses of moisture, lipid, protein, ash, fiber, etc....
02 May 2020 5,807 0 View
Here is the germane citation: Calvin J Gordon, Egor P Tchesnokov, Emma Woolner, Jason K Perry, Joy Y. Feng, Danielle P Porter, Matthias Gotte. Remdesivir is a direct-acting antiviral that...
15 April 2020 3,785 17 View
I am trying to validate a Biotin-streptavidin pull down protocol. My downstream application is Western blotting. I am working with HEK-293t cells and BASU. I incubate with streptavidin magnetic...
27 March 2020 5,935 7 View
In general biophysical experiments are carried out maintaining pH of around 7. For this purpose one can use various buffer systems like phosphate buffer, citrate buffer, tris buffer, bicarbonate...
24 March 2020 3,702 3 View
Hello, I would like inquire on the process of extraction of protein contents from the green pea by means of decantation. What I know is that a decantation is a separation process in which...
23 March 2020 705 4 View
I have an important information to share n my article , the reference of that is a news article. Can you guide me how can i cite that in text and in detail?
21 March 2020 6,790 6 View
I need to produce formazan to make a standard curve, (as in this paper:Quantitative Determination of Superoxide in Plant Leaves Usi... ) The reaction requires riboflavin and methionine,...
06 March 2020 6,535 3 View
I use modified Ellman's method for determine cholinesterase inhibitory activities of some compounds. But i suspect that some thiourea derivetives form thiol group and give false negative yellow...
06 March 2020 1,461 5 View
ATP energy-storing molecule as is no doubt an efficient! as not only it is used as energy storage and provider in all forms of life and has probably been around for billions of years. Even in the...
09 February 2020 9,173 6 View
Sometimes I need to centrifuge reactions containing enzymes, however, I've realized I have absolutely no idea if spinning very fast could damage the enzymes. Does anyone have any data on safe...
02 February 2020 7,392 5 View
Hello everybody, I am trying to calculate the irreducible representation of point group 3m (C3v) for NBT (having R3c space group - 161). Could you please give some clarification in this regard?
01 January 2020 6,035 2 View
looking for some best ref. books on Viva in Clinical Biochemistry / Biochemical Analysis / Biochemistry PDF ??
12 December 2019 4,619 4 View
Lysozyme is consisted of 4 disulfide bridges while the number of disulfide bridges for BSA is 17. Nevertheless, lysozyme is known to be a more rigid protein. What are the other factors affecting...
12 December 2019 1,291 4 View
what is mass-based and volume-based energy storage capacity of ATP and typical carbohydrates? Is it possible for an autotroph to be evolved/found that convert sunlight into energy of ATP, store...
11 November 2019 9,754 2 View