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Questions related to Biochemistry
i am cloning my gene of interest in vector pet32 and pgex4t. upon transformation i got colony on antibiotic containing LB Agar. also colony pcr and pcr with the plasmid extracted from the clone...
23 April 2023 1,125 4 View
Normmally you would go from pFastBac to DH10Bac or paceBac to Multibac. I currently have paceBac and DH10Bac and want to avoid ordering multibac if possible. Thanks!
21 April 2023 6,297 2 View
I have been working on B. subtilis transformation for a while, and despite many efforts and the use of different protocols I have not succeeded in doing it so far. I used the well known protocol...
17 April 2023 5,567 2 View
I've tried a transformation with a gene from a wild strain, however, it seens that this gene is toxic for my bacteria, I need information about gene toxicity when you try to modificate a wild...
11 April 2023 3,659 0 View
i am facing problem in adding kollman charge to my protein so that i added gasteiger charge. is this ok. will this effect binding affinity or binding energy?
04 April 2023 2,189 0 View
We have lost our melt calibration file for HRM analysis during software update and can't use precision melt analysis software for data analysis. We are using R programming based analysis for HRM....
28 March 2023 2,280 0 View
i have to use chloroform to measure the absorbance by Tecan plate reader but i can not do by using Polystyrene microplates (due to incompatibility of chloroform with Polystyrene). please guide me...
27 March 2023 2,069 0 View
Hi I am working with human bone marrow derived stem cell. I am trying to lyse and wash the RBCs in the sample. Any suggestions to what to use and if there is an exact protocol of how to do it?...
26 March 2023 6,404 1 View
I am using GROMOS96 43A1 for MD simulation. So far I had been using PRODRG server to generate topology files of small-molecules , then reaching the mentioned server is being troublesome and not...
25 March 2023 4,835 0 View
I have tried to determine selenium in UV-Vis Spectrophotometer by using Methylene blue but it is very sensitive to time with in 30-60 sec i hv to measure and after that the color is vanished. so...
24 March 2023 9,891 7 View
i need to react chromium and selenium in hydrothermal conditions
22 March 2023 6,154 1 View
I mean, is it possible to rely on the RF - factor , for example, by comparing samples that contain toxins and the RF of the standard Toxin ?
11 March 2023 2,794 2 View
02 March 2023 9,209 0 View
I've been trying to synthesize NH2-HA recently, but it failed. Can someone please tell me why and how I can improve it? The synthesis steps were as follows: I dissolved 500mg of HA in ddH2O and...
02 March 2023 2,783 5 View
Elution buffer composition: 50mM Tris-Hcl(pH9.0) + 10mM reduced glutathione + MilliQ water
01 March 2023 8,192 2 View
I was reading a few articals for my research on TiO2 used in paint formulation, and I found only few extenders like Calcined kaolin, Hollow spheres, Barium sulphate that could reduce by little the...
24 February 2023 2,450 1 View
This was from a whole cell lysis protocol using 125uL of Y-PER and 2uLof PIC. I agitated the yeast cell pellet twice and then centrifuged the samples. I added 10uL of 2X loading buffer+BME with...
22 February 2023 9,470 1 View
Hello, I bought this antibody to make electrochemical immunosensors, I bought the ab93903 antigen which is recommended. I prepared the antibody to the desired concentration in 10mM PBS pH 7.4 and...
22 February 2023 7,164 3 View
I did check its solubility properties and I found the value of 400 mg/mL = 400 g/L, but despite that, I always observe some crystals in the solution i'm preparing, and it only as a 6 % w/v...
15 February 2023 4,522 1 View
Hello, I want to isolate the outer membrane protein from a bacterium that I grew under different conditions and display it on sds gel. Sonication device was used in all the articles i found as a...
09 February 2023 9,204 7 View
Suppose if I input "STAT3", is there any database that can show expression changes in STAT3 from different proteomics experiment datasets, in a tabular form (assuming these datasets have been...
07 February 2023 6,521 0 View
Hi everyone, I work with Arabidopsis thaliana. I want to know what is the best strategy to strore plant saplings after the treatment to preserve the quality of RNA (at least for a week). 1. Is it...
04 February 2023 5,454 4 View
hello i am in need for ideas on this question so as i can accomplish my term paper, enlighten please....
01 February 2023 9,947 6 View
I am trying to prepare a Minimal Media with lower and higher pH than usual. I had success with the pH 3 media but the higher pH one is giving me trouble. Right now the media...
11 January 2023 6,508 4 View