Dear all,
For some applications in AUC, we would like to correctly determine the partial specific volume of our biomolecules.
We currently use Sednterp as a principal approach which diplays a theoretical value.
Is someone has a nice protocole to share to calculate partial specific volume on a densitometer? We use a DMA 5000 Tm as a densitometer.
Best,
Sébastien
Engelbert Buxbaum
The most precise way to determine \bar{\nu} for small volumes and molar concentrations is to measure the natural frequencies of a U-shaped tube filled with blank buffer and protein solution (see doi:10.1016/S0006-3495(98)77735-5).
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