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Questions related to Biochemistry
For telomere elongation, 2 μL of telomerase extract with various concentrations ranging from 101 to 106 cells was incubated with 1 μL TS primer (100pmol) in 10 μL reaction buffer (50mM Tris-HCl,...
20 April 2022 9,382 2 View
I have seen a couple of panel data research where the authors fill up missing data of a variable with similar variable from another source. For example , getting inequality data from WDI and...
30 January 2022 4,471 7 View
Activation of telomerase enzyme to increase telomere length in humans.
17 January 2022 9,747 4 View
How to increase the solubility of ammonium molybdate tetrahydrate in water... Kindly suggest any other aqueous solutions.
11 January 2022 5,027 3 View
Uv spectra for the following isatin derived molecule (which I have used as ligand) was obtained in the region 273nm due to π → π* transitions of aromatic rings and 308nm may be due to n → π∗...
09 January 2022 1,222 5 View
DTT, b-ME and TCEP are commonly used in biochemistry for the reduction of cysteins. Recently, I investiged the effect of the oxidative state of polymer-binding peptides on their binding behaviour....
05 January 2022 9,376 10 View
I am interested to do Virtual Screening for Plant products. Please suggest me tools to carryout the virtual screening.. Thanks in Advance...
03 January 2022 1,436 8 View
Dear Experts, How do I write a netcdf file with VMD from a netcdf/dcd trajectory file? It seems that VMD can read a netcdf file but cannot write it, as follows: Info) Using plugin netcdf for...
23 December 2021 1,243 0 View
We are interested to detect somatic mutation in serum/plasma of brain tumor patients, in samples collected after resection and before start of adjuvant therapy. Is it feasible to get the somatic...
22 December 2021 2,008 5 View
In our two -year field experiment, white clover pure stand resulted in higher biomass yield compared to its respective mixtures with grasses and as well as with the mixture of red clover with...
22 December 2021 716 3 View
As per literature LOD is calculated using equation LOD = k (Sy/S). Where, Sy is standard deviation of blank value, S is the sensitivity of immunosensor, and k (k=3) is the confidence level factor....
16 December 2021 3,253 3 View
HELLO! I WORK IN A COMPANY THAT IS WORKS IN AVICULTURE BREEDING, BUT NOW, IT HAS SOME INVESTMENTS IN THE AQUACULTURE FIELD AND I GOT THE CHANCE TO BE A SENIOR PARTNER IN A NEW PROJECT WHICH IS...
08 December 2021 6,508 3 View
The alkyl or aryl based ligands/passivating agents are frequently used for the passivation of metal nanoparticles or colloidal suspension during liquid-phase synthesis. How much should be the...
06 December 2021 5,959 3 View
Dear community, i am currently trying to create min. volatility portfolios including several indexes. My issue is that i can't just cut the historic stock returns at the last point where all...
16 November 2021 6,106 1 View
I occasionally have to measure protein concentrations. For this I use the BCA Protein Assay Kit by ThermoFisher. I never use all of the standards. Is there a way to store them, so I can reuse them...
27 October 2021 4,348 0 View
I would like to know a details on how to use it, method to rinse net, variation in size, and method to preserve sample(plankton).
25 October 2021 9,985 4 View
I Need the formula to calculate amylase activity from bacillus. Can please someone guide me on how can I do that?
23 September 2021 4,863 1 View
The result that i obtained from acetylation process showing that the resistant starch value is higher compared to the amylose content. Theoretically, from understanding is that resistant starch...
18 September 2021 8,543 5 View
Hello. I hope someone can help me or give me an idea on how to prepare HEPES buffer with Chelex-100 using the statement I have read on a published journal. Enyzme (500nM) was incubated with...
25 August 2021 6,216 4 View
We have a spectrophotometer (Tecan Infiniti) for a 96-well plate, but I may also have access to a traditional cuvette spectrophotometer.
19 August 2021 1,962 3 View
It was noticed that floating behaviour of animals as a major variable in swim endurance studies. Any suggestions are welcome to avoid the same. We have come with a plan to keep a vibrator / ripple...
04 August 2021 3,585 0 View
It is almost two and half a months that I have started callus induction, co-cultivation and Agrobacterium mediated transformation in three different rice varities. Approximately three weeks ago I...
23 July 2021 7,657 3 View
hi in fermentation of a-amylase after some hour my ph increase and my broth to be gel form, my guess is my bacteria lysis, Why does this happen?
10 July 2021 3,661 3 View
I was wondering whether anyone has experience buying individual reagents instead of the agilent kit for seahorse. I'm looking to run a glycolytic stress test and was concerned that Agilent uses a...
07 July 2021 6,614 3 View