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Questions related to Biochemistry
In a textbook (Biochemistry, 2nd edition by C. Grisham and R. H. Garrett) I found the brief description of so-called calcisomes. These are vesicles/organelles that store calcium, similar to the...
03 March 2018 1,060 2 View
I have a Bachelor degree in Pharmaceutical Sciences, a diploma in advanced Biochemistry and a Master degree in stem cells and regenerative medicine. I would very much like your help in suggesting...
03 March 2018 5,419 4 View
Most cancers are detected when they cause symptoms that lead to medical evaluation. Unfortunately, in too many cases this results in diagnosis of cancers that are locally invasive or already...
28 February 2018 8,055 8 View
I want to calculate the Total phenolic content ,so i am going to prepare a standard callibration curve of gallic acid at different concentration. which concentration should i select?
24 February 2018 8,557 6 View
I performed deletion mutagenesis along the c terminal region of a protein targeting two specific leucine residues, and showed no change compared to wild type, as assayed by dual...
06 February 2018 4,450 2 View
02 February 2018 7,487 4 View
I want to get the Km and Kcat of enzyme E3. However its substrate C is very unstable for its R-S conformation exchange.
02 February 2018 3,965 4 View
02 February 2018 2,679 4 View
Myonuclei acquired by overload exercise precede hypertrophy ... In case of sarcoplasmatic hypertrophy, I have the following proposol. In relation to hypertrophy, we speak about increase of...
02 February 2018 3,854 1 View
Dear researchers, I want to know about torsion angles in nucleotide. Can someone help me?
01 January 2018 3,079 0 View
I want to extract curcumin and, if it's not pure, I won't be able to use the spectrophotometer in order to prove the presence of curcumin in the extract, because the existence of other substances...
19 December 2017 291 9 View
I prepared graphene oxide by modified Hummer's method. I would like to synthesize graphene from graphene oxide for a biosensor. but nearly every article is also performing graphene synthesis using...
19 December 2017 7,682 4 View
During the last year, a patient with desminopathy (Thr341Pro DES mutation) was found to have a 1.8 to 20-fold decrease in the level of circulating immune complexes, a 1.3-fold decrease in...
19 December 2017 5,036 0 View
12 December 2017 4,577 5 View
12 December 2017 7,937 10 View
How can we compare stability of two proteins with the help of NMR spectroscopy? what are the traditional NMR techniques that we use for this purpose?
12 December 2017 3,716 4 View
Hello, I am conducting a research on the key domains of a different protein sequences. Can you give me the link of the best software that I can use to do this task ?
12 December 2017 5,784 3 View
There is many generally accepted facts such as the neurons and the cardiomyopathy being some of the most highly metabolically active cells. But I struggle to find any studies comparing these...
28 November 2017 5,862 0 View
Hi dear researchers In a nature report "Amperometric inhibitive biosensor based on horseradish peroxidasenanoporous gold for sulfide determination" measuring sulfide concentration in a wastewater...
19 November 2017 8,427 3 View
I could see 660 nm and 416 nm in different papers. I have doubt that in this study whether we measure the amount of protein which is not denatured or some other content. If the absorbance gives...
11 November 2017 3,418 5 View
11 November 2017 5,194 3 View
07 November 2017 2,564 4 View
Hello everyone, My question is the next : Why the DHB matrix is a reducing agent while sDHB matrix is not a reducing agent? In this article it is written that sDHB is a non reducing agent Fast...
20 October 2017 273 3 View
Can we use reversed micelles method to extract alpha glucosidase? I want to extract alpha glucosidase from Saccharomyces cerevisiae and Bacillus spp. by using reversed micelles technique. But I...
10 October 2017 2,369 2 View