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Questions related to Biochemistry
In a scenario, where I have about 25 protein sequences of a lytic enzyme (Variants) of bacterial origin, I would like to estimate the mutational distance of each of these sequences and...
26 December 2022 9,692 1 View
The topic of my project is an immunosensor. For that, I dropped casted antibody with nanomaterial on the working electrode, then I kept the electrode at 4 c, but I had to keep that electrode at...
06 December 2022 2,770 4 View
during last 2 year, i have not obtain root from my transgenic poplar plantlets. I previously found the regeneration protocol and obtained regenerated transgenic plants. however, when i tried...
29 November 2022 6,304 0 View
I am carrying out a CRISPR knockout using PX458 plasmid with the following information GROWTH IN BACTERIA Ampicillin, 100 μg/mLBacterial Resistance(s) 37°CGrowth Temperature Stbl3Growth...
07 November 2022 5,478 2 View
Hello for everyone I come to you to help me find a solution to my problem in the GC MSD I did the tune the first time and everything was good after I injected a sample the filament goes out during...
07 November 2022 6,306 4 View
I'm synthesising Cu nanoparticles with EG, PVP, and ascorbic acid. How long the particles will be stable in EG before washing them using a centrifuge?
04 November 2022 1,127 2 View
I am trying to express and purify nanobodies from a nanobody library in E. coli. I am using a pBAD vector to which I added the pelB sequence for periplasmic localization. The nanobody is fused to...
02 November 2022 9,859 3 View
The swissADME tool can calculate iLOGP for a compound. What pH is this measured at?
28 October 2022 5,499 2 View
I am trying to examine the cytotoxicity of an antioxidant agent on undifferentiated SH-SY5Y cells. In my previous try, all of the doses I tried gave results above 100% and all the wells were...
22 October 2022 6,998 3 View
How to estimate the minimum number of subjects that should be enrolled for phase 1 of a clinical trial? The objective of phase 1 is to test for the safety, side effects, dose etc. I know we...
14 October 2022 5,321 5 View
I am trying to perform a biochemical assay which requires me to take readings for reduction in NADH levels at 340 nm. Could anyone kindly suggest what plates should I be using. I currently have...
13 October 2022 771 2 View
I used unlabeled cells (first image) to set the gate and the second image is my positive control. I am looking for percentage of green cells (FL-1, ''expressing'' cells in my experiment) that are...
11 October 2022 9,022 2 View
Genome sequence data need to be converted into numerical database to perform machine learning algorithm.
11 October 2022 8,424 4 View
I have a published phylogenetic tree but no access to the rough data used to generate the tree. I want to compare among clades in terms of morphological characters. How do I include the...
18 September 2022 9,381 1 View
So we do microscale thermophoresis (MST) with labeled protein and labeled DNA. Typically standard titrations are easily performed and there are no problems. However, with some experimental...
16 September 2022 8,785 2 View
Hi, everybody I'm currently producing manganese peroxidase and I need to assay its activity. I'm planning to use the Wariishi's method, it requires to prepare sodium malonate buffer 100mM pH 4.5....
15 September 2022 3,293 0 View
I am using an oncolytic virus for virotherapy and want to determine the MOI of the virus to treat the cancer cell line. I have attenuated the virus in the form of the vaccine, can anyone please...
10 September 2022 8,967 0 View
Dear scientists Please inform me which is more convenient centrifuge for chitosan modification? Please suggest the possibility. Thank you
17 August 2022 1,997 0 View
Does anyone know why the peak of purified his tag protein look like this?
03 August 2022 5,151 2 View
I have been trying to do regeneration of rockmelon plant using cotyledonary explant. After a few years of trying, I'm not getting the shoots. I'm able to get up to the callus stage but the callus...
25 July 2022 240 0 View
Tried 0.5-1 mg/ml IAA for rooting but no roots
22 July 2022 2,934 0 View
We are trying to use buffy coat stored in RNALater at -80 Deg for RNA extraction. However, we are unable to get sufficient RNA. Looks like the leucocyte lysis is not happening properly. Any...
21 July 2022 9,116 0 View
Dear colleagues, I perform neutrophil isolation with percoll from blood sampling to perform RNA sequencing but when I verify RNA quality (using Agilent RNA 6000 nano), all my RIN are under 5....
04 July 2022 7,968 0 View
I recently made a backup of my data from Geneious prime and now the files are not as a .zip or.geneious etc but as G files. They are not importing into the Geneious Prime software. Does anyone...
22 June 2022 1,738 0 View