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Questions related to Biochemistry
Hi all, I am wondering how I can combine the trajectory in GROMACS. I used the coding 'gmx trjcat -s xx.tpr -f xx-1.xtc xx-2.xtc xx-3.xtc -o xxx-combined.xtc' and 'gmx trjcat -s xx.tpr -f...
17 September 2023 2,594 0 View
Hi please can anyone guide me on how to retrieve clonal complex (CC) from MLST sequence of seven house keeping genes of E. Coli, and also to do spartial distribution of the CC. Also, due to...
15 September 2023 5,284 1 View
dose titration , dose escalation
14 September 2023 1,299 1 View
My nanopore sequencing run has generated some ''unclassified reads''. Can anyone explain what causes them to be unclassified and how to avoid them in future?
14 September 2023 1,422 3 View
I wish to study dye degradation using these nanoparticles but I am unable to find adequate research papers on biosynthesis of iron-selenide nanoparticles.
09 September 2023 10,021 4 View
There are different methods to determine LD50 of chemicals and xenobiotics, these include Lorke's method, up and down method, Karber's method, Reed and Muench etc. But which of those is relatively...
02 September 2023 2,745 0 View
Does anybody have a reliable method of being able to measure increased microbial biomass during a ready biodegradation test (e.g. OECD 301F) between the beginning and the end of the test (or even...
29 August 2023 7,569 6 View
Are there any historical, spatial datasets available for different Agriculture Market Prices / Crop Prices from around 1950 or 1970 onwards? > FAO has missing data and mostly very courser scale...
29 August 2023 8,600 0 View
Why does water have a high boiling point and surface tension and type of bond is responsible for water molecules sticking to other water molecules?
28 August 2023 9,692 0 View
I have been through the various paper where mostly the works of Chan has been cited. Does anyone have the laboratory protocols in the determination of very labile, moderately labile, less labile...
24 August 2023 1,136 0 View
I am trying to extract DNA from skin swabs of Salamandra atra but the DNA concentrations I get are very, very low (like < 0,5 ng/µl). I am using the Qiagen QIAamp DNA Micro Kit in combination with...
23 August 2023 2,595 2 View
Hi all! I recently started performing enzymatic treatment on Nannochloropsis oceanica in order to disrupt the cell wall. Throughout the different extracellular components I want to analyze, there...
22 August 2023 6,164 1 View
How many PBMCs can I seeded for 5 days in a 96-well plate in 200 µl RPMI with FBS and PHA?I started with 30,000, and I don't know if it's not enough
14 August 2023 7,478 1 View
I am trying to develop Curcumin bioconjugates by esterifying -OH moieties of Curcumin aromatic rings. By performing UV spectrophotometric analysis of the conjugate (red curve) in a mixture...
02 August 2023 5,245 2 View
We have an oldschool PPMS equipment with ACMS option, and recently got the problem with DC measurements. When starting a measurement it returns: Measuring DC Extraction 8/1/2023 10:54:37...
01 August 2023 4,277 1 View
OK
31 July 2023 6,549 2 View
Please explain an experimental technique to determine the presence of free radicals generates from the rotten edible oils.
26 July 2023 4,115 2 View
I have synthesis by mixing 10 mM AgNo3 and curry leaf extract
25 July 2023 657 1 View
Is there a tool that can output the most correlated/inversely co-related gene combinations from TCGA dataset? I mean WITHOUT AN INPUT GENE. Just give the highest R value combinations from TCGA...
14 July 2023 5,803 0 View
Are there any standards/ limits for respiratory deposition dose like this much microgram of Pm 2.5 is applicable for 1 hr exposure/ if any....I read many papers but I couldn't find it...Kindly...
13 July 2023 8,157 3 View
I would like to do an experiment blocking TNF to reduce neuroinflammation.
10 July 2023 8,171 3 View
The background of my Western Blots is very uneven. I use premade NUPAGE gels and PVDF membrane. For blocking I using intercept blocking buffer for 1 hour at room temperature. Primary antibody is...
03 July 2023 3,530 3 View
Sir, can anyone explain the DSC curve of biomass
26 June 2023 5,988 1 View
Hello all, My goal is to crystallize RNA-bound HIV-I Integrase. But once I mix equimolar amounts of RNA: protein (100uM), it immediately turns cloudy. Buffer of the protein is 20mM HEPES 7.5,...
23 June 2023 7,600 2 View