Sakshi Saini

Yes, you can calculate the binding efficiency of a dye with a biopolymer using UV-Vis absorption spectroscopy, provided that the dye has a characteristic absorbance peak that is not interfered with by the polymer.

How to Calculate Dye Binding Efficiency (Simply):

Calculate the Binding Efficiency

If:

• Dtotal= total dye added (µmol or mg)
• Dfree= unbound dye measured from supernatant
• Dbound=Dtotal−Dfree

Then:

Binding Efficiency (%) = Dbound/Dtotal×100

Example:

Let’s say you started with 1.0 mg dye, and after separation, you found 0.2 mg unbound dye (by UV).

Then:

• Bound dye = 1.0 – 0.2 = 0.8 mg
• Binding Efficiency = (0.8/1.0)×100=80%

Thank you so much, sir, for a detailed explanation Md. Rehan Alam

I want to ask one more question ,as the dye concentration increases then can we suppose that the binding efficiency will decrease?

Sakshi Saini

Sorry for the delay,

You have asked me a very good question.

Yes, in most cases, if you keep the amount of biopolymer (binding sites) fixed and increase dye concentration, then the binding efficiency (percentage of dye bound) decreases.

Here’s why:

• At low dye concentration, almost all dye molecules can find free binding sites → high binding efficiency (close to 100%).
• As dye concentration increases, the finite number of sites becomes saturated → extra dye remains unbound in solution.
• Thus, the absolute amount of dye bound increases, but the fraction (binding efficiency = bound dye / total dye × 100%) usually decreases.

Thankyou so much sir