Hello. How should I proceed with atf-3 ifstaining? Hello. I was going to try with ATF-3 and NEUN in drg in the Oxaliplatin Cipn model. But atf-3 doesn't express in neun. And I also see several papers showing that atf-3 goes round in the nucleus, and my results seem to be attached to the outside.

1. Nf200, cgrp, ib4 split and watch with atf-3

2. Atf-3 is dyed wrong.

3. Change the triton x-100

I've thought of two solutions. I'd appreciate it if you could give me any suggestions on which one would be better. blocking buffer : 4%bsa+10%ngs+tritonx-100 0.3%+0.9%nacl 1차 buffer : 1% bsa+5%ngs+tritonx-100 0.3%+0.9%nacl

I attached confocal data. green : neun, red : atf-3, dapi Thank you.