What can be a loading control for subcellular fractions?

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Krutika Thakkur

In my lab we use for mitochondria: MRPL12, CLPP

cytoplasm: GAPDH, calnexin

nucleus: Histone (?)

Payel Das

I want to check whether the loading of lysates from each fraction are equal.

Is there any common protein that is expressed in all the fractions?

Hi Cornelius

Thank you very much for your suggestion.

I also think this is not possible. But I was asked this question. Thats why I was wondering what opinion of other researchers is.

I think we can only understand the localization by analyzing the loading controls of specific subcellular fractions.