I am using a chloroform: methanol (2:1) solution as a lipid extractant for my insect cuticle sample (femur) mounted on UV glue. However, the method I am using is fairly rudimentary-

I wet a small square piece of lens cleaning tissue with the solvent mixture and then manually rub it onto the sample. I wait for about 60 seconds and then repeat this a couple of times. Finally, I rub the cuticle once again with a dry tissue. It is worth noting that I am not currently interested in extracting the lipid, but rather in removing the lipids. However, the method that I am using is not controlled, and since my sample is quite small (about 1 to 3 mm long), I also run the risk of contaminating the mounting medium (UV glue). Furthermore, the sample is mounted on a special setup (Broadly speaking, it is mounted on a tiny beam using UV glue, and that beam is attached to a larger beam, which is a part of my setup. It is important that I don't dismount the sample during the lipid removal process, so centrifuging is not an option.)

To do this in a more controlled manner, I use a microcapillary pipette to ensure that I use the same volume of solvent every time.

Would you be able to suggest a protocol for a controlled and effective method of lipid removal?

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