I want to detect the genes responsible for resistance of Klebsiella pnuemoniae, Klebsiella oxytoca, Enterobacter cloacae, and Pseudomonas aeruginosa resistance to streptomycin. I need the primer sequence(s) for the isolation of these genes.
Target Gene
Primer Name
Primer Sequence (5′→3′)
Amplicon Size
Reference
aadA
aadA-F
GTGGATGGCGGCCTGAAGCC
525 bp
Madsen et al., 2012
aadA
aadA-R
AATGCCCAGTCGGCAGCG
strA-strB
strA-strB-F
ATGGTGGACCCTAAAACTCT
891 bp
Gebreyes, and Altier, 2002
strA-strB
strA-strB-R
CGTCTAGGATCGAGACAAAG
rpsL
rpsLF
GGCATGGCCGACAAACAGAACG
501 bp
Jagielski et al., 2014; Xia et al., 2017
rpsL
rpsLR
ACTGGGTGACCAACTGCGATCC
rrs
rrsF
TGGCCATGCTCTTGATGC
1707 bp
Jagielski et al., 2014
rrs
rrsR
CGCCCACTACAGACAAGAAC
gidB
gidBF
GGAGTGCGTAATGTCTCC
785 bp
Jagielski et al., 2014
gidB
gidBR
GTCGGTGGTGTCATTTCC
@Anthony Ayodeji Adegoke Thank you so much. Could you please provide the full name of the articles you referenced?
Ganiyat Bukola Abdulganiyu Many researchers have used the primers. Simply search using the sequences if you cannot find the listed authors. See the attachment as well
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