One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms... | Contact experts in Bacteria to get answers
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Questions related to Bacteria
i want to grow rhizosphere bacteria on nutrient broth and subsequently check their growth through OD. I need a robust protocol that can tell me all the details about conducting this experiment.
24 January 2020 4,850 3 View
I have been working on creating on knocking out the ThyA gene (an essential gene) in e. coli using lambda red but have only been successful with lab type strains (MG1655 and BW25113), but...
09 January 2020 2,685 4 View
Could someone suggests which MW of FITC-Dextran is used for chemotaxis of bacteria? Also, if I make a FITC-dextran solution in PBS then how long can we store this solution at 4 degree Celsius?
01 January 2020 3,653 1 View
01 January 2020 5,520 3 View
I am working on a project where I am testing the resistance of a certain bacteria to copper and was testing it by adding copper at varying concentrations to r2a agar. However, despite sitting in a...
01 January 2020 901 5 View
I recently ran some 16S sequencing data using the DADA2 pipeline using the silva database (silva_nr_v132_train_set.fa) for taxonomic assignment. Strangely, the genus Polaromonas sp. is being...
01 January 2020 2,450 4 View
01 January 2020 4,781 3 View
Metagenome sequencing data analysis, Bioinformatics
23 December 2019 5,941 15 View
Hi researchers, I've been working with my own mucin obtained from porcine's gut and I'm trying to purify it by my own. The thing is that when I do dilutions of the fecal sample, and inoculate...
18 December 2019 1,784 0 View
Can biofilms be seen by pathologists when they do lab studies? For example, if a human has a biofilm in their colon and does a colonoscopy with biopsies sent to the pathologist, could the doctor...
12 December 2019 9,581 5 View
Hello all, We have a discussion in our lab and would love your input: Is it possible for cultured bacteria that are in, or approaching, their stationary phase (in our case OD 5 - 6) to go back...
12 December 2019 3,091 8 View
Hi, I am trying to fit my experimental adsorption data to pseudo second order linear equation. the fit is good (R2 > 0.9988) but it is giving me negative intercept from which I will have to...
28 November 2019 3,017 4 View
Hello everyone, Can anybody let me know how to conjugate PEG with bacteria?
27 November 2019 213 3 View
Hello everybody, we are working on the eradication of Helicobacter pylori and inhibition of its biofilm. We are looking for the suggestions that what compounds would be better to use against H....
18 November 2019 9,320 3 View
Hello, I've set an inverted microscope setup on optic table to see bacteria on 0.13-0.17 cover slip with short WD objective(0.17) but I just could see them once and have difficulties to regulate...
11 November 2019 3,238 1 View
I was measuring ATP concentration in E. coli for one of my research project. I saw some literature mentioning ATP concentration in E. coli as 1-5mM. However, I am getting much less (in...
11 November 2019 9,325 6 View
11 November 2019 1,770 3 View
11 November 2019 1,481 4 View
We recently obtained several cyanobacterial strains (Synechocystis, Chroococcus, Synechococcus, etc.) from the culture collection in Göttingen. Upon first inoculation in BG-11 (UTEX recipe),...
30 October 2019 5,966 5 View
Hi, I would like to know the general range of permeability (P = D*k/L where D diffusion constant, k partition coefficient, L cell wall + cell membrane thickness) of antibiotics across...
10 October 2019 4,193 3 View
In the qualitative method, I used tributyrin agar plates and phenol red agar plates (contained 0.1% oil) to differentiate esterases and lipases. Because my target enzyme is lipase. Strains showed...
10 October 2019 1,383 6 View
There are two interpretive criteria for the results of epidemiological in-vitro anti-bacterial sensitivity studies, namely EUCAST and CLSI. Can I use both in a single study - e.g. using CLSI in an...
10 October 2019 6,944 4 View
Hi. Is there a reliable protocol / method / kit for quantification of capsular polysaccharide in Acinetobacter baumannii or any other bacteria for that matter? preferably without the use of...
26 September 2019 7,372 3 View
I want to isolate DNA and RNA from faecal and blood samples in order to determine which pathogens (viruses, bacteria, fungi, parasites...) are present in the samples. I currently have one PCR...
22 September 2019 7,837 4 View