One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms... | Contact experts in Bacteria to get answers
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Questions related to Bacteria
Do bacteria grow in soil that remains under water all the time? Or, are bacteria growing under water all anaerobes? If not, where from do they get O2 if there is not much agitation for O2 to be...
18 January 2019 6,731 7 View
We are planning on doing microbiome analyses of raw milk and would like to know which microbial DNA extraction method we should use?
09 January 2019 5,466 4 View
Looking for standardization protocol for bacterial CFU count using SYBR Green dye. It will be appreciated for the help if someone who works on the same.
01 January 2019 5,404 4 View
Hi, i'm trying to isolate many fungal strains, but the strains are contaminated wiht bacteria; i get Gentamicin (VIAL OF 2 ML WITH 160 mg OF GENTAMICIN SULFATE) my question is... wich is the best...
01 January 2019 8,537 9 View
I'm culturing hiPSCs on matrigel, and these square shaped entities appeared in the center of the well plate. They don't move when plate is shaken, I'm not sure what this is.
01 January 2019 5,785 5 View
At the lab we've been culturing V. Indigofera (strain: NRRL B-3162), but we can't get it to produce indigoidine. We've tried growing it on TSA, PCA, and LB agar, incubating at 25° C. Even well...
01 January 2019 1,838 4 View
01 January 2019 3,650 7 View
I would like to have more details on the microencapsulation techniques the most suitable for protecting Lactobacillus bacterias during the digestion. What are the experimental protocols ? What are...
01 January 2019 9,084 0 View
Dear all, I tear one's hair out with my experiment. In the lab, we have validated whole blood activation with heat killed bacteria. we have seen an increase of inflammatory cytokine in...
01 January 2019 1,901 3 View
I need a container of titanium tetrachloride (TiCl4) which works in the range of 700-1000°C. which kind of material can resist this condition and doesnt get corroded?
20 December 2018 2,560 6 View
Mannitol Salt Agar is the most common technique used to differentiate CoPS from CoNS. But I am curious to know any other techniques or media which can be used to differentiate CoPS from CoNS on an...
12 December 2018 8,555 27 View
In my experiment I have checked the bacterial mutation frequency after different treatment. The treated bacteria were spread in a plate containing an antimicrobial. The mutation frequency was...
12 December 2018 5,825 4 View
Are there techniques other than SEM to confirm bacterial outer membrane vesicles. Actually, our SEM is out of order for some time, so, I want to confirm them by some other techniques.
12 December 2018 9,564 4 View
I am trying to identify bacteria on skin that can utilise lactic acid as carbon source. So i intend to swab skin and then grow bacteria on minimal media supplemented with lactic acid. My question...
12 December 2018 3,526 0 View
Hi, I have a product being lyophilized, and would like to know what aspects of formulation (Not the cycle parameters) decide how much water content will be left out post freeze drying. Any leads...
13 November 2018 6,953 4 View
I am looking for recommendations to effectively culture pesticide resistant bacteria. I tried with normal LB agar without pesticides and results are not satisfying as only one particular...
11 November 2018 6,624 7 View
Bacteria reduction is generally represented in log scale varying from 1 log reduction onward upward 9 log. However, this only represent 99.99999... reduction. So how to represent 100 % bacteria...
11 November 2018 7,365 5 View
CFU/mL of sample can easily be determined using solid medium. However, difficulty is always encountered using broth culture in spectrophotometer which may be considered more appropriate by some...
11 November 2018 1,776 12 View
How can we prove that the staphylococcus aureus is apoptosis?
11 November 2018 9,313 2 View
My vesicle samples get constantly contaminated by these strange microorganisms, that unfortunately I'm not able to identify. Does anyone know which species is it and how to avoid them? Our samples...
11 November 2018 2,411 6 View
05 November 2018 6,994 11 View
02 November 2018 257 6 View
Our lab have Staphylococcus aureus ATCC 25423 and I study other papers that use it. But now we can not find it in ATCC site. What did happen?
10 October 2018 5,084 0 View
i am working in a project where we want to separate the magneto-tactic bacteria from the Egyptian aquatic environment and use the magnetosomes of these bacteria after that what is the technique...
10 October 2018 5,685 6 View