Lyophilized Antibodies for Microfluidic Device?

I am using monoclonal lyophilized antibodies for a microfluidic chip which I would like to be stored at room temperature. What are the easiest methods to reactivate these antibodies for...

31 March 2018 8,941 2 View

What the best method for Research about Fruit Flies (Bactrocera spp)?

Entomology, Phytopathology, Integrated Pest Management

05 March 2017 6,708 14 View

How do i measure body size/image incongruence?

Hi. I am looking for a way to measure body size or body image incongruence. i would like to try and measure the differnece between actual and perceived body size. i was wondering if anyone knows...

11 December 2016 6,056 8 View

Are there any specific antibodies or markers for M phase of cell cycle?

07 December 2016 4,014 3 View

Can anyone recommend a good dissociation questionnaire, other than the DES?

i am looking for a good dissociation questionnaire, other than the DES. i would like something that had been validated and has been cited often.

10 November 2015 1,929 7 View

Why do dividing RPTECs show two different cluster morphologies?

We are currently culturing immortalized renal proximal tubules (RPTEC/TERT1) at passage 1. The cells are adherent, and so grow in clusters, but strangely, we observe two different types of...

24 June 2014 5,073 2 View

Does leaving (dilute) trypsin in with the cells during passage slow their growth?

I am growing hCMEC/d3 cells in Lonza EGM media with Bulletkit supplements. The passage protocol I use calls for just directly adding the resuspended cells, trypsin (.25% in EDTA) and all, to the...

03 March 2014 6,717 5 View

Apakah mahasiswa bisa dilibatkan dalam project ini?

saya mahasiswa tingkat akhir yang ingin mencari project penelitian untuk tugas akhir , saya adalah mahasiswa fisika saya tertarik dengan remote sensing pemetaan dan tomografi, mungkin jika...

01 January 1970 7,177 1 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

How can a reference point be fixed to an attachment point in Abaqus?

I am currently trying to copy an assembly feature, that is present in an old version of the model I am using, onto a new model. The feature is a reference point that is fixed to an attachment...

16 February 2021 8,872 2 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

Room Occupancy Calculation ?

15 February 2021 1,997 6 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

Plasmid amplification in S2 cell?

When co-transfect S2 cell with selection vector (pCoHygro or pCoBlast ) to make a stable cell line, does the gene integrate into the genome or just like E.coli? How do the plasmids amplify within...

09 February 2021 5,423 1 View

Would using an asRNA for repressing transcription of a high expression Listeria monocytogenes non-coding RNA harbored in a plasmid be a viable option?

Hello all, I am interested in deleting a highly expressed non-coding RNA located on a Listeria monocytogenes plasmid. Currently, there are not any optimized gene deletion methods for Listeria...

09 February 2021 4,994 1 View

Review Papers regarding transcriptional regulations & promoter types/classes in Gram positive bacteria?

Dear fellow researchers, Could you please suggest me excellent review papers discussing transcriptional regulations and/or promoter classes in Gram positive bacteria? Thank you so much for your help!

08 February 2021 6,909 3 View