Hello
I am infecting cells with bacteria in a well and then I am doing serial dilution of this well 1 in 10 and culture the two last dilution on MHA agar
I have difficulty in getting accurate number of bacteria if I do it in triplicate
Could anyone suggest me hint or tipe for this technique
Thank you
For me to count bacterial colonies I always use the Miles and Mizra method and it is true that it is outdated but very accurate for counting bacterial colonies.
If you do a google search for 'serial dilution and the standard plate count' you will get multiple sites with explanations and videos that demonstrate the procedures.
I agree using Miles and Misra although very traditional is the recognised process. Ensure you start with a 0.5 McFarland turbidity suspension. Use calibrated pipettes and 20ul drops of suspensions. It has worked for decades Good Luck
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