Hi,

I am looking for an assay (simple and fast) to quantify a 12AA peptide quantification inside PLGA nanoparticles.

I did a PLGA/peptide solution in DMSO and after that I used F127 as surfactant.

I quantified by measuring at 205 and 280nm. As calibration curve, I used the free peptide. The obtained standard curve was good, but the quantification of peptide inside the PLGA did not work.

Does anyone know another method to quantify a short peptide into PLGA nanoparticles?

Thank you