One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms... | Contact experts in Bacteria to get answers
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Questions related to Bacteria
Hi all, I recently did a nanopore run using the ligation sequencing kit with native barcoding. I had a high amount of reads and data generated. The biggest issue I have is that when I...
10 August 2022 3,065 3 View
I need to prepare different molar concentrations 5 mM, 10 mM, 15mM, 20mM and 25 mM solution from 30% H2O2 solution. These solutions concentrations will be applied on cotton leaves
17 May 2022 2,460 0 View
I am currently studying the properties of Al2O3 films through Atomic Layer Deposition, and I use Aluminum contacts for my MIM Structures. However I noticed that when I put a piece of Si Wafer and...
09 May 2022 591 5 View
And the bump was seen for a longer time with increasing in the dopant concentration. I am working on LaMnO3 with Bi doping. below is the graph attached for the same, Thank you
04 May 2022 7,742 2 View
To determine the concentration of glucose, I'm using the DNS assay which is an easy and fast way. Now, I'm looking for an assay where I can measure the concentration of glycerol. A colorimetric...
24 February 2022 7,127 0 View
I have prepared a fungal enzyme extract in Citrate phosphate buffer pH 5. The sample was also frozen in -20 one night and was used in Christ Alpha 1-2 LD plus. After 5 hours find the sample is...
19 November 2021 8,545 0 View
The protein is in phosphate buffer pH 8 and when i try to decrease to ph7 it aggregates. Is a virus envelope protein with 42 Kda and it is fusogenic. I want to do some NMR experiments with this...
18 November 2021 1,366 13 View
I am currently working doing a lot of N/N 2D gel electrophoresis for investigating replication structures in yeast. We might invest in a bead beater, which can handle a specific volume, but I am...
22 September 2021 2,651 4 View
Hi, I am looking for an assay (simple and fast) to quantify a 12AA peptide quantification inside PLGA nanoparticles. I did a PLGA/peptide solution in DMSO and after that I used F127 as surfactant....
21 September 2021 3,550 1 View
I have encountered complete blockage of needle valves used for TiCl4 (in chemical vapor deposition). Despite heating of valve, the needle valve is completely blocked and does not open even with...
15 September 2021 2,225 3 View
we use mycorrhiza to improve nutrient use efficiency and root enhancement, and phosphate solubilizing bacteria to help solubilize the phosphate and make it available to plants. Can both of them be...
24 June 2021 2,093 4 View
I recently sent some RNA lyophilized samples to a sequencing facility. As the samples were lyophilized, I can sent them at room temperature and I did not expect any degradation. However, they did...
11 June 2021 1,444 0 View
I have multiple models (hobby Sisyphean app: https://snwikaij.shinyapps.io/RF_macrophyte_models/) predicting (at least trying) presence/absence of riverine macrophytes. I would like to include...
03 June 2021 2,370 0 View
I am studying algae-associated symbionts and their ecological importance. I know that bacteria and archaea can contribute nutrients to the host. But can fungi (not fungi-associated...
13 May 2021 7,137 5 View
I am looking for a simple assay to estimate the before and after amounts of protein (%) for extracted chitin. If someone can explain the calculation for the related technique, that would be...
16 April 2021 7,965 4 View
Hi, I'm currently working on an edible mushroom. I ground a fruiting body but cannot get a fine powder. As I know, mushroom with fruiting body has started its maturity phase. Thus, the fruiting...
05 April 2021 242 8 View
hi I want to synthesize nanoparticle PLGA-PEG carrying doxorubicin and also some nucleic acid (mirna). Between Doxorubicin (hydrophobic form) or Doxorubicin HCL ( hydrophilic form) which one...
12 March 2021 6,491 5 View
The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....
28 February 2021 4,970 2 View
Hi, I would like to learn about monitoring macrophytes in rivers, lakes, and heavily modified water bodies. As much as I know, macrophytes are monitored in rivers and lakes, but they are not used...
24 February 2021 5,942 12 View
I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...
17 February 2021 8,939 5 View
We planned to collect macrophytes from a river where aquatic plants are of high density. What is the suitable method of sampling.
06 February 2021 4,320 3 View
Hello I am infecting cells with bacteria in a well and then I am doing serial dilution of this well 1 in 10 and culture the two last dilution on MHA agar I have difficulty in getting accurate...
27 January 2021 9,502 5 View
I want to examine a kind of fruit's extract's antimicrobial effects. At first I want to put it under the UV radiation to prevent the mold or any kind of fungi's and bacteria's growth. The...
25 January 2021 8,666 20 View
Hi, I want to inject (inoculate) a special bacteria to a laboratory mouse. Could you please guide me if you know any useful method?!
16 December 2020 1,638 5 View