One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms... | Contact experts in Bacteria to get answers
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Questions related to Bacteria
I am trying to go for HPTLC fingerprinting and quantification of an herba extract , however the Standard I need is very expensive (phylproof Reference Material) but there is the same cheaper...
05 November 2022 2,686 2 View
I am standardizing a latex agglutination test for detecting WSSV with my specific antibody. But agglutination reaction is not evident. I am sure about my antibody; hence, I think it is not...
27 October 2022 810 2 View
At the beginning of cell culture and in the log phase, despite the considerable need for energy and ATP, cells deliberately convert glucose to lactate, and the amount of lactate usually increases....
21 September 2022 4,919 2 View
I prepare 0.2 w% of Al2O3+CuO hybrid nanofluid in Water+EG (60:40), after 1 hour and 30 minutes of sonication about 50 ml of fluid just get disappeared. No signs of splashing were there, even...
18 August 2022 9,169 3 View
Hi all, I recently did a nanopore run using the ligation sequencing kit with native barcoding. I had a high amount of reads and data generated. The biggest issue I have is that when I...
10 August 2022 2,978 3 View
I need to prepare different molar concentrations 5 mM, 10 mM, 15mM, 20mM and 25 mM solution from 30% H2O2 solution. These solutions concentrations will be applied on cotton leaves
17 May 2022 2,402 0 View
I am studying algae-associated symbionts and their ecological importance. I know that bacteria and archaea can contribute nutrients to the host. But can fungi (not fungi-associated...
13 May 2021 7,055 5 View
Hi, I'm currently working on an edible mushroom. I ground a fruiting body but cannot get a fine powder. As I know, mushroom with fruiting body has started its maturity phase. Thus, the fruiting...
05 April 2021 170 8 View
The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....
28 February 2021 4,713 2 View
I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...
17 February 2021 8,722 5 View
Hello I am infecting cells with bacteria in a well and then I am doing serial dilution of this well 1 in 10 and culture the two last dilution on MHA agar I have difficulty in getting accurate...
27 January 2021 9,371 5 View
I want to examine a kind of fruit's extract's antimicrobial effects. At first I want to put it under the UV radiation to prevent the mold or any kind of fungi's and bacteria's growth. The...
25 January 2021 8,492 20 View
Hi, I want to inject (inoculate) a special bacteria to a laboratory mouse. Could you please guide me if you know any useful method?!
16 December 2020 1,531 5 View
After the liophilization process, the RNA is degraded. I tried to fix the problem by washing everything with DEPC treated water and freezing the samples with liquid nitrogen before lyophilizing....
12 December 2020 5,097 4 View
Carrion Ecology: What is the best way the statistically analyse the correlation of bacteria community data to volatile profiles and to fly community data across 3 time points using ordination...
25 November 2020 5,938 3 View
Dear all, Good day! Recently i tried to ferment my blended soya bean powder mixed with Natto bacteria culture in a Natto maker (containing some water) at around 45 degree celsius overnight....
24 November 2020 7,258 20 View
Could anyone give valuable inputs of this strain nomenclature F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, ΔpotD783::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514?. Actually I need only pot D mutant...
24 November 2020 6,406 5 View
I have to measure the growth of the bacteria in a piece of fabric for my senior design project. I don't know how to measure it. The only way that I know is by the naked eye or by the microscope....
24 November 2020 3,037 6 View
We would like to analyse thousands of E. coli genomes and identify orthologous genes. Before this we would like to filter the bad quality genomes by BUSCO (https://busco.ezlab.org/). What % of...
16 November 2020 5,241 3 View
How can i get nonsporulating bacterial bacillus subtillis strain Δ spoIIE without antibiotic growth conditions ? This ΔspoIIE::erm strain is showing growth conditions with antibiotic...
11 November 2020 7,299 4 View
Is there any difference in the life span of current variants of this evolutionarily ancient phototrophic bacteria and the pre-historic ones.?
12 October 2020 6,976 5 View
I am lay in this subject. Is DNA sequencing the best way to identify the different types of soil bacteria present in soil samples? Ideally, I'd like to find a technique that allows me to identify...
30 September 2020 4,734 9 View
Looking for a Article that investigate improvement of geotechincal properties of acidic soil/sand using microbially induced calcite precipitation (MICP).
25 September 2020 4,037 1 View
Hi, I am wondering what is the best experiment (cheap and fast) to screen co-expression of a list of genes in different bacteria strains? Lets say, we have around 100 bacterial strains and for...
11 August 2020 7,682 3 View