After the liophilization process, the RNA is degraded. I tried to fix the problem by washing everything with DEPC treated water and freezing the samples with liquid nitrogen before lyophilizing. But it continues to degrade.
Dear
I have attached some articles and link. I hope they would be useful.
https://patents.google.com/patent/CN106591432A/en
https://patents.google.com/patent/WO2016165831A1/en
Kindly check https://pubmed.ncbi.nlm.nih.gov/27515991/
Also check https://academic.oup.com/jpe/article-pdf/10/1/81/17711695/rtw042.pdf
Thank You so much for the information!
I found in some papers that carbohydrates or some lipids can be used as lyoprotectants (for avoid RNA degradation during lyophilization). But I have not found information about whether this...
12 December 2020 3,443 0 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
I am puzzled about the properties of gelatin, when used for tissue-embedding. Hard gelatin can be melt again by temperatures about 40°C. Formalin-fixed gelatin is like crosslinked protein and...
07 August 2024 1,686 1 View
The paper in question is "Interpolation of Nitrogen Fertilizer Use in Canada from Fertilizer Use Surveys". This paper was very recently published by Agronomy (MDPI). Agronomy has, in the last day...
07 August 2024 9,934 3 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
Do these peaks in the curve indicate primer dimer or non-specific products?
01 August 2024 7,466 0 View