One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms... | Contact experts in Bacteria to get answers
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Questions related to Bacteria
I've been working with plant bacteria and investigating their ability to produce siderophores. For a qualitative test I chose a method described hereModified microplate method for rapid and...
27 July 2020 7,282 4 View
I would like to do a transformation with E. coli ATCC 25922. Would like to simply add a plasmid for amp. resistance. I need to use ATCC 25922 for my research question. Does anyone have a protocol...
24 July 2020 5,535 1 View
I am co-culturing bacterial strains with human cells (WBCs) and there are about 3000 different bacterial strains. The idea is to carry out flow cytometry experiments with these samples (in 96 well...
10 July 2020 3,222 5 View
Answer related to metabolism of bacteria would be appreciated.
24 May 2020 4,168 2 View
I‘m confused that why there are hundreds of flagella grown on some bacteria. It are unlikely used only for movement,but what is the real function of it?
28 April 2020 6,381 1 View
Dear all, I have some bacteria DNA partial sequences (16s rRNA). They sequenced only by 9 F primer. Their length is under 1100 bp. When I draw phylogenetic trees for some of them by MEGA, my...
24 April 2020 9,287 8 View
Is there any explanation for this that why the same specie of bacteria is qourum sensing positive when it is isolated from clinical sample but when we isolate that specie from marine sediment, it...
01 April 2020 3,130 4 View
I have sequenced various bacterial genomes, and what I need to know is whether these isolates are clonal or not. There are several percentages of similarity among these isolates, yet I do not know...
01 April 2020 9,845 10 View
Hello, Many protein shake powder mixes contain digestive enzymes such as protease, lipase, and cellulase. However, the products do not detail from which organism the proteins are produced or...
22 March 2020 5,151 3 View
I'm working with both facultative anaerobes and obligate aerobes. I was wondering do SIM and/or MIU tests work if the bacteria are aerobic? If not what tests should I do in order to find out...
20 March 2020 455 4 View
I am conducting DNA extraction experiments from K562 cells. For a reason unbeknown to me, the total mass of DNA extracted happens to be much larger than what I expect considering the number of...
16 March 2020 6,354 3 View
I am considering to use PCR for identification of X. vesicatoria (from tomato spot), however I am still exploring alternative tests. I have found an ELISA test for several Xanthomonas species, but...
16 March 2020 1,588 7 View
I already tested three different sequences and had problems with dimers and melt curve (low specificity). Any reliable primers? I'm studying rat microbiota with qPCR.
13 March 2020 7,751 2 View
I have not seen this type of bacteria morphology before. I am wondering what it might be and how to classify it. Is it even bacteria? Could it be fungi?
12 March 2020 4,484 6 View
The question does not require much explanation. Interested to know if there is some study on overall pattern of temperature range of permanent inactivity of various viruses and bacteria of all...
11 March 2020 8,876 44 View
I am struggling to find a helpful protocol for detection of these two bacterial species (one originating from tomato wilt disease and the other from tomato spot disease). I am unsure of how to...
10 March 2020 8,855 5 View
I'm having extreme difficulty identifying this bacteria... here are the results of some of the tests I've performed. Any insight is greatly appreciated! TTC Motility: strongly motile Gram stain:...
05 March 2020 2,300 6 View
Hello, I'm currently working on DNA extraction from E. coli suspended in PBS. I'm working with concentrations ranging from 102 CFU/mL to 105 CFU/mL (and 108 CFU/mL as a positive control) and I...
03 March 2020 4,105 3 View
Hello all, I am planning to culture bacteria B.subtilis (strain 1085) for my research and was looking for the availability of cells in Sigma Aldrich or Merck. Unfortunately, I couldn't find it....
28 February 2020 1,107 7 View
Working on comparative genome analysis on Zika virus, having a minor issue while working with genome workbench? Anyone done this before, who can guide me on this? Actually, couldn't able to...
26 February 2020 4,521 2 View
This is my first time working with thioglycollate broth and based on everything I've found I am not a 100% sure what this type of growth represents. I can see a growth on the top, then a line of...
06 February 2020 8,250 7 View
I'm trying to isloate bacteria from rhizosphere, kindly share some robust protocols that i can use for isolation.
02 February 2020 9,624 3 View
For reducing the antinutritional factors of plant feed ingredients bacterial fermentation is a good option. But I wish to know among the different strains of bacteria which one may be a better option.
02 February 2020 526 2 View
02 February 2020 7,870 4 View