One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms... | Contact experts in Bacteria to get answers
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Questions related to Bacteria
After the liophilization process, the RNA is degraded. I tried to fix the problem by washing everything with DEPC treated water and freezing the samples with liquid nitrogen before lyophilizing....
12 December 2020 5,147 4 View
Carrion Ecology: What is the best way the statistically analyse the correlation of bacteria community data to volatile profiles and to fly community data across 3 time points using ordination...
25 November 2020 6,094 3 View
Dear all, Good day! Recently i tried to ferment my blended soya bean powder mixed with Natto bacteria culture in a Natto maker (containing some water) at around 45 degree celsius overnight....
24 November 2020 7,504 20 View
Could anyone give valuable inputs of this strain nomenclature F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, ΔpotD783::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514?. Actually I need only pot D mutant...
24 November 2020 6,534 5 View
I have to measure the growth of the bacteria in a piece of fabric for my senior design project. I don't know how to measure it. The only way that I know is by the naked eye or by the microscope....
24 November 2020 3,295 6 View
We would like to analyse thousands of E. coli genomes and identify orthologous genes. Before this we would like to filter the bad quality genomes by BUSCO (https://busco.ezlab.org/). What % of...
16 November 2020 5,341 3 View
How can i get nonsporulating bacterial bacillus subtillis strain Δ spoIIE without antibiotic growth conditions ? This ΔspoIIE::erm strain is showing growth conditions with antibiotic...
11 November 2020 7,405 4 View
Is there any difference in the life span of current variants of this evolutionarily ancient phototrophic bacteria and the pre-historic ones.?
12 October 2020 7,108 5 View
I automatically built a GSMM using ModelSEED and retrieved all the metabolites that appear in the model using cobrapy. Now I'd like to convert the ModelSEED IDs that appear in the model to KEGG...
06 October 2020 3,659 0 View
I am lay in this subject. Is DNA sequencing the best way to identify the different types of soil bacteria present in soil samples? Ideally, I'd like to find a technique that allows me to identify...
30 September 2020 4,854 9 View
Looking for a Article that investigate improvement of geotechincal properties of acidic soil/sand using microbially induced calcite precipitation (MICP).
25 September 2020 4,087 1 View
I want to siliconize my cell culture bioreactor vessel, and buying sigmacoat is a little expensive by considering the other materials which I need and my budget. So I was thinking if it's ok to...
06 September 2020 8,001 2 View
Hi, I am wondering what is the best experiment (cheap and fast) to screen co-expression of a list of genes in different bacteria strains? Lets say, we have around 100 bacterial strains and for...
11 August 2020 7,774 3 View
Sample dilution is done on some complex matrice prior to chromatographic analysis whilst other complex samples are analyzed directly with no sample dilution. what is the significance of sample...
31 July 2020 7,088 10 View
I've been working with plant bacteria and investigating their ability to produce siderophores. For a qualitative test I chose a method described hereModified microplate method for rapid and...
27 July 2020 7,411 4 View
I would like to do a transformation with E. coli ATCC 25922. Would like to simply add a plasmid for amp. resistance. I need to use ATCC 25922 for my research question. Does anyone have a protocol...
24 July 2020 5,596 1 View
Yogurt and its benefits
15 July 2020 4,512 97 View
I am co-culturing bacterial strains with human cells (WBCs) and there are about 3000 different bacterial strains. The idea is to carry out flow cytometry experiments with these samples (in 96 well...
10 July 2020 3,352 5 View
Tell me can I prefer MFT Or MPN
03 July 2020 2,931 2 View
I am doing large-scale fermentations and have significant problems with bacterial contamination which I suspect are lactic acid bacteria. I would like to add an antibiotic that will not affect the...
24 June 2020 7,754 1 View
We can obtain high environmental significance throughout microbial degradation of petroleum hydrocarbon contaminants which depends on the indigenous microorganisms to convert or mineralize the...
05 June 2020 7,191 15 View
I have Agrobacterium (AGL1) harboring pBI121 which contain gene of interest, it has been utilized in callus transformation, now i want confirm the transformation in early stage to ensure further...
04 June 2020 6,381 6 View
I am interested to calculate LOD and LOQ by calibration method.
27 May 2020 896 17 View
Answer related to metabolism of bacteria would be appreciated.
24 May 2020 4,313 2 View