Hello every one
I Have some problems with tissue wash off when im trying to IHC. I tried some adhesive slides but I didnt get result. my sample is trachea with a lot of cartilages. has some body any advice to keep it more adherent.?
First, step of thawing the slide is very critical. Place slide flat horizontal, not in coplin jar, let the OCT melt for 5- 10 minutes. Than wash the sections by pouring PBS around section. Idea is to remove OCT from under the section. If there is thin layer of OCT left between section and slide, then section tend to slip. Do 4 washings very gently, so that Whole OCT under the section is removed. Then section will settle down and will stick to the slide. Also use slides which are suitable for cryo. Now a days, good quality slides are available from many compnies. Be patient at this first step. Generally we ignore first step. Keep slides flat always.
I use Vectabond coating for samples with cartilage. There are similar products from other companies. https://vectorlabs.com/products/vectabond-reagent-tissue-section-adhesion/
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