Defining mixed model with repeated measures data?

HI all, I have been working with daily diaries data ( 6 emotions of which 3 positive and 3 negative) were assessed for 30 days. These observations (1level data) are then nested within subjects...

13 February 2021 1,810 3 View

High percentage agarose gel for better resolution?

Hi all, it might be a very basic question, but I need some opinions: I need to separate a 294 bp wild type PCR product from a 232 bp knock-out PCR product. I want to do DNA extraction out of the...

08 June 2020 9,557 9 View

What do you think of the impact of Covid-19 on faster acceptance of innovative healthcare technologies?

After this pandemic outbreak, nothing will be the same. Previously suspicious attitude toward accepting innovative solutions in clinical practice and long times for regulatory procedures and...

09 April 2020 7,691 10 View

Any experience with Health Informatics Journal?

We would like to publish something which is in the domain of Health Informatics, but several Journals have almost identical names. any suggestions, experience with publishing with those Journals...

22 February 2020 7,635 9 View

Do we need a standardization prior to publication of any machine learning application?

What do you think about so high accuracies reported in so many publications especially in medicine and psychiatry? Talking about overfitting and so frequent lack of internal and external...

07 October 2019 2,558 4 View

Is it possible to stain cells with Acridine orange/ethidium bromide in hydrogel 3D invasion assay?

I am planning to perform 3D invasion assay with spheroids in hydrogel and to visualise live/dead cells. Is it possible to visualise cells with AO/EB (besides CAM/EtHD) dyes? Thanks

18 October 2018 5,722 3 View

Can LPS be captivated by the tube if you freeze and subsequently thaw it?

15 January 2017 7,051 3 View

Does someone can recommend a good Elisa kit for mouse NTx (N-terminal telopeptide ) ?

Hello everyone, I need to measure NTx (N-terminal telopeptide) marker in a mouse serum. I would really appreciate if someone could share experience with this kind of ELISA kit, most importantly...

10 April 2016 5,458 1 View

What is wrong with my intestinal organoids?

I am trying to grow swine intestinal organoids from crypt preps. I have been doing it with mouse tissue for at least a year now. For the pigs, a few days after getting the crypts, there are...

05 April 2016 3,294 7 View

Does anyone know how to create nucleotide and amino acid deduced sequence visualization in an automated way?

I would like to generate a visualization similar to that you can see in the attached image (Mu et al., 2012, Fish and Shellfish Immunology Journal).

15 September 2012 7,903 6 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

No title

02 March 2021 3,060 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View