the fungal tested is fusarium oxysporum and the plant extract i used for treatment is tridax procumbens. i have no idea why there's a lot of white dot appear. is it contamination from bacteria? pls help me 😭
Phil Geis oh because there's some research paper that stated that the plant extract I used (tridax procumbens) have antifungal activity, but so far I haven't found research paper that use t. procumbens on f. oxysporum. is it possible that different fungal could have different antifungal effect is being tested on the same plant extract?
There could be several reasons why you did not observe a zone of inhibition in your antifungal test against Fusarium. Here are some possible explanations:
1. Ineffective antifungal agent: The antifungal agent you used might not be effective against Fusarium. Different fungal species have varying susceptibilities to different antifungal compounds. It is possible that the specific agent you used does not effectively inhibit the growth of Fusarium.
2. Insufficient concentration: The concentration of the antifungal agent you used may have been too low to produce a measurable zone of inhibition. Antifungal agents are typically used at specific concentrations to ensure effective inhibition. If the concentration is too low, it may not be sufficient to inhibit the growth of Fusarium.
3. Inappropriate testing method: The testing method you used may not be appropriate for Fusarium. Different fungi have different growth characteristics and may require specific testing methods. It is important to use a suitable method for testing the antifungal activity against Fusarium.
4. Inoculum density: The density of the fungal inoculum you used might have been too high. A high inoculum density can overwhelm the inhibitory effect of the antifungal agent, making it difficult to observe a zone of inhibition. It is important to use an appropriate inoculum density to ensure accurate results.
5. Time of observation: The duration of your experiment might not have been sufficient to observe a zone of inhibition. Some antifungal agents require a longer incubation period to exert their inhibitory effects. It is possible that a longer incubation period is needed to observe a visible zone of inhibition.
6. Fungal resistance: It is also possible that the Fusarium strain you tested is inherently resistant to the antifungal agent you used. Fungal strains can develop resistance to specific antifungal compounds over time, and this could be a contributing factor to the lack of a zone of inhibition.
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