Do FAP natural inhibitors exist ?

More Philippe Mellet's questions See All

Coulkd you help finding a paper about Bryophytes ?

Some monthes ago on RG I found a nice paper by a bryologist explaining that in a single small tuff of epiphytic mosses different species might co-occur. I remember a plate illustrating type...

01 July 2024 5,102 0 View

What tag to use inside the sequence of an hydrophobic transmembrane protein ?

Hi everyone, I want to study the translation efficiency of multiple version of the same protein coded by different genotypes. To do so I need to conserve the translation initiation context ->...

29 April 2024 6,779 1 View

What if all multilayered plastic packagings could become recyclable? Come to the keynote of P Shahgaldian at # ISPTS 2024?

plastic revolution?

10 April 2024 4,191 2 View

What is the shortest sequence encoding a fluorescent protein ?

Hi everyone, everything is in the title question ! To the best of your knowledge, what is the shortest sequence you know that translate into a fluorescent protein that could be used for flow...

07 March 2024 1,762 1 View

Who has experience with transfecting HaCaT keratinocytes ?

Hi everyone, I'm currently working on the HaCaT keratinocyte cell line. I have them in culture under low and high calcium concentration to keep them undifferenciated or differenciated,...

04 March 2024 2,153 0 View

Why the concentration of my expression vector drop with time and how to stop it ?

Hello everyone, I use several pcDNA3.1 expression vectors to transfect cells. The vectors were prepared by midi-prep a year ago and diluted in TE buffer. Now that I run new experiments, I...

04 March 2024 1,867 3 View

MODFLOW: How to efficiently modify existing 3DGrid using GMS?

I work on a MODFLOW Model using GMS to simulate subsurface water exchanges between a small straightened stream and its heavily modified alluvial plain. I’m looking for efficient methods to modify...

09 January 2024 708 0 View

How to get rid of chelex resin before 0.22µm filtration ?

Hello everyone, I'm curently working on HaCaT cells which require low calcium medium to return to undifferentiate state. To do so, I need to chelate my FBS for 1h with chelex resin among other...

12 December 2023 4,975 2 View

Can you share scientific literature or research experience about building a timeline with participants with interviews?

In an action-research, our research team is conducting interviews to identify key moments about professional practices in the field of education with participants.

04 December 2023 2,114 1 View

Does 5'UTR length can influence alternative splicing ?

Hello every one, I'm working on the in vitro expression regulation of a viral gene. I'm looking at the splicing efficiency of several versions of the gene (comming from different genotypes). To...

10 September 2023 9,689 1 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

What is the prepared reference material that can be used in inductively coupled plasma optical emission spectrometry (ICP-OES) to measure REE and U ?

What is the prepared reference material that can be used in the ICPE-9820 Shimadzu Japan instrument, which employs inductively coupled plasma optical emission spectrometry (ICP-OES) to measure...

06 August 2024 1,896 1 View

Could dyes amplify the spectrum of light to a specific wavelength?

I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...

05 August 2024 3,290 1 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Is artifacts in XPS possible to build high deviation in binding energy larger than 5 eV??

Hello. Thanks for your consideration to see my question. Recently, I conducted XPS anaylsis of g-CN that is prepared from thermal polycondensation of DCDA, so-called conventional bulk-g-CN,...

30 July 2024 9,824 2 View

Tips for teaching Orton Gillingham+ to students with autism?

I am beginning to use OG+ in my district and noticed during training there are a lot of materials and routines, which may be overwhelming for my students, especially those on the autism spectrum....

30 July 2024 5,695 0 View

Can i use the protease inhibitors during cell membrane vesicle preparation ？?

I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...

30 July 2024 7,077 1 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

Culture fibroblasts with or without FGF2?

I'm new to primary human fibroblast culture and I'm studying aging using cells from old and young donors. Some research groups have used media supplemented with FGF2, so I cultured cells in the...

29 July 2024 3,030 2 View