What is the best way to extract RNA from skeletal muscle?

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What is saturation solubility of insulin in saliva?? I need it to know sink condition ?

05 August 2024 4,407 0 View

What is sodium bicarbonate solution concentration for extraction in lab ?

13 July 2024 2,909 2 View

What is the exact procedure and materials for converting from ester to carbolic acid by base hydrolysis??

11 July 2024 4,645 3 View

Can we do purification by separatory funnel instead of column chromatography ? My solvent is acetone and the purification solvents are two..?

08 July 2024 6,928 4 View

How can I dry emulsion (there are some non dissolve substance in my organic solution ) by Na2SO4? How can I separate my non dissolved material ?

26 June 2024 9,122 5 View

How to evaporate DMF from solution? Knowing that rotavap doesn’t work. Could anyone help?

23 June 2024 1,858 0 View

How to visualize a cell palett of a small cell amount?

Have you ever seen a small number of cells, 50-100 thousand, after centrifugation as a dry pellet in an Eppendorf tube without a microscope? Are there methods to potentially stain the cells in the...

09 June 2024 6,311 0 View

Handling BrainVision matlab files with ECG data in EEGLAB?

I have 217 preprocessed BrainVision matlab files (.mat) with ECG data in them. I've been having trouble creating a study for these. I know BioSig works for ECG data, but it doesn't seem to work...

21 April 2024 1,028 2 View

EEGLAB - how to import multiple .mat files from different folders all at once?

I have 25 folders for 25 participants, all with .mat files inside that are preprocessed. Most of them have the same amount of .mat files - a couple participants didn't finish the study, so they...

17 April 2024 1,897 3 View

How the concept of oblivion is interwoven within psychoanalytic theory ?

24 February 2024 5,581 0 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

Swimming/space travel depends on the proprioceptive muscle spindles?

When the entire neocortex is ablated in rodents, although they are still able to swim, all the limbs move continuously and asynchronously (Vanderwolf 2006; Vanderwolf et al. 1978). Normal animals...

03 August 2024 835 3 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View