40 Questions 54 Answers 0 Followers
Questions related from Syed Zahid Ali Shah
If anyone had the new impact factor list for 2018 please share with me... Many Regards
06 June 2018 7,548 11 View
I tried to cite some articles but some of them are not correct? i mean when more than three authors then in some cases it shows et al., but with many it shows full authors names no matter how many...
12 December 2017 3,534 4 View
I gave prion infection to hamsters and now i did Lamin A/C stain for brain Slides? more or less expression of Lamin A/C indicates?
12 December 2017 4,400 0 View
I have TEM images of brains from infected hamsters? how can i differentiate stressed mitochondrion from normal healthy ones? any tips? Thanks
11 November 2017 7,395 4 View
I just noticed on main page of many impact factored journals the retracted articles? What could be the cause? The ones i saw were mostly Chinese authors...
11 November 2017 6,335 0 View
If anyone has the new list of current impact factor and five years impact then please share with me for the 2016-17 list? Many regards
06 June 2017 4,567 6 View
Can somebody please help me finding articles that are associated with longterm usage of FK506 resulting in toxicity? if animal model results of neurodegenerative disorders had toxicity it will be...
06 June 2016 10,068 2 View
I wanna check Calcineurin expression Via western blot for Calcineurin A Fragment and Calcineurin B fragment...will both of these fragments expression on western blot will be high or low as the...
04 April 2016 4,564 0 View
i have some pallets left after centrifugation of brain homogenates made in RIPA buffer. Now if i want to see NF Kb p65 nuclear translocation status in these samples, is it ok to use Urea? or any...
04 April 2016 6,757 2 View
Can anyone tell me the best protocol which can help me visualizing Nfkb P65 through confocal microscopy or any other IHC protocol in prion infected paraffin embedded section?
04 April 2016 1,386 2 View
i did some IHC staining on brain sections of prion infected hamsters...some slides showing overexpression and some underexpression on nuclear protein Lamin A/C? what does it mean in simple words?
04 April 2016 5,722 5 View
Can anyone tell me what are the fluorescent cells visible after thioflavin-S all around cerebellum of hamsters but they are only visible in the cerebellum of prion infected animals...in control...
04 April 2016 9,082 4 View
wel...l is there any easy way through which i can statistically analyze the videos that i made for memory tests specially i wanna check locomotion and rearing activity with a the central novel...
03 March 2016 8,565 5 View
i just want to know the accuracy of calcineurin antibody result and calcineurin colorimetric assay for brain tissue homogenates....and if i do western blot for calcineurin A and B subunits they...
03 March 2016 5,424 2 View
i wanna know if 263k strain can infect cell lines like N2a or HSSY5Y, if yes then can i use brain homogenate of infected hamsters to infect these cell lines? and what would be the best dose if i...
02 February 2016 1,408 2 View
i read somewhere if i know the concentration of each primary antibody of my interest i can just incubate all the PVDF membrane with a solution containing all the antibodies in specific...
02 February 2016 3,138 4 View
can anyone help me what brain homogenate concentration would be better if i want to detect Total CREB, pCREB, Total BAD and pBAD through WB from brain tissue of hamsters infected with Prion 263K?
01 January 2016 3,906 6 View
i want to do calcineurin activity tests on brain homogenates, first i wanted to use an ELISA type of kit but i dont have ultrahigh centrifuge...so alternative could be if i use antibody for PP2B...
01 January 2016 1,199 4 View
i have a very simple question that if i want to see the effect of Salubrinal and tauroursodeoxycholic acid (TUDC) in growth of reproductive cells for example in growth of embryo in vitro...will it...
12 December 2015 6,514 5 View
I wanna purify RML strain infected brain of mouse for prion Prpsc....does anybody know approximate yield from one brain of terminally ill mouse?
12 December 2015 6,128 8 View
Can anybody suggest which software is easy and user friendly if i want to count Neurons, Astrocytes and Microglia in Slide images after Immunohistochemistry?
11 November 2015 3,168 1 View
i just want to know if anyone knows the safe dose of minocycline that can be used in neuroblastoma cell line such as N2A? i did use in vivo but dont know much about invitro use...will be thankful...
11 November 2015 5,964 2 View
If I want to purify prion protein scrapie (PrpSc) from brain tissue of mouse and in the first step I need to put protease inhibitor cocktail in PBS, then homogenize brain in it. I have 100x...
11 November 2015 4,876 6 View
If the primary antibody is raised in same species can I use multiple antibodies in same IHC slide? For example if I want to see neurons, astrocytes and microglia on same slide. All the antibodies...
10 October 2015 3,798 8 View
it takes long time till the slides reach to room temperature after heat induced antigen retrieval so can i cool them down in refrigerator?
10 October 2015 8,496 5 View
if i made ten percent brain homogenate and i want to add lysis buffer suppose RIPA buffer...what should be the ratio of PBS made homogenate to RIPA buffer? thanks
09 September 2015 6,926 2 View
if i want to detect Prpsc through WB, then i need to make brain homogenate and sonicate it first and centrifuged at 10K x g, but whether i will put PK after centrifugation or before? i personally...
08 August 2015 8,519 2 View
i wonder how people make such nice pictures and diagrams in their research papers? is there any useful free to download and easy to use software? Many regards
07 July 2015 8,489 1 View
i found an ELISA kit to detect pCREB and total CREB from Abcam company...can i use it for brain homogenate? as i know brain tissue homogenate is too dirty?can anyone help or suggest...
05 May 2015 855 3 View
I gave hamsters prion infection about 40 days ago. So with 263 strain signs should appear after approximately 60 plus days. Now, one of the hamsters is having movement problems in forelegs and it...
05 May 2015 5,532 4 View
I want to see neurodegeneration in my hamsters infected with Prpsc...can anybody guide me how can i easily see either apoptotic markers in brain homogenate or any fixed tissue techniques? thanks...
05 May 2015 9,519 6 View
can anybody suggest some behavioural studies that i can do in syrian hamsters?i have control and prion infected groups?any memory analysis tests?
04 April 2015 9,530 2 View
Can anyone suggest some Antibiotics that alters calcineurin levels in brain?or any drug that can change levels of pCREB and pBAD levels in brain?
04 April 2015 5,193 2 View
i want to detect stress produced by prion strain in SH-SY5Y or N2A but i am not sure which cell line is better?can anybody help me with selection of cell line?
03 March 2015 4,887 4 View
Primery cells are better or N2Tera cells are better for axoplasmic reticulum related calcium surge studies in axon degeneration?
12 December 2014 5,963 1 View
I want to know about calpain1 and calpain2 inhibition via calpastatin in vitro but i want to know more about calcium influx inhibition as a tool for axonal degeneration reversal mechanism?Possible...
11 November 2014 8,445 1 View
i would be thankful if somebody can let me know what are the best neuron cells that i can use in vitro for axonopathy studies caused by prion(prp106-126)?how long this peptide will take to cause...
11 November 2014 6,486 0 View
Can anyone give a suggestion about the prion infection in N2a cell line to check the efficacy of metformin? is it better to use a fragment of prion 106-126 for cell viability and other analysis or...
01 January 1970 5,994 0 View
What could be the possible molecular pathway or trigger behind reduced AMPK activation in prion infected animals via PP2A/C? Does it goes back to calcium as trigger from ER?
01 January 1970 4,844 0 View
I wanna ask if i can store the remaining part of 10% brain homogenate at -80 centigrade for later use? I had bit extra homogenate so i wondered if i can use it for later use? Homogenate is made in...
01 January 1970 4,527 5 View
