i would be thankful if somebody can let me know what are the best neuron cells that i can use in vitro for axonopathy studies caused by prion(prp106-126)?how long this peptide will take to cause degeneration?
If anyone had the new impact factor list for 2018 please share with me... Many Regards
05 June 2018 7,596 11 View
I gave prion infection to hamsters and now i did Lamin A/C stain for brain Slides? more or less expression of Lamin A/C indicates?
11 December 2017 4,457 0 View
I tried to cite some articles but some of them are not correct? i mean when more than three authors then in some cases it shows et al., but with many it shows full authors names no matter how many...
11 December 2017 3,590 4 View
I have TEM images of brains from infected hamsters? how can i differentiate stressed mitochondrion from normal healthy ones? any tips? Thanks
10 November 2017 7,458 4 View
I just noticed on main page of many impact factored journals the retracted articles? What could be the cause? The ones i saw were mostly Chinese authors...
10 November 2017 6,383 0 View
If anyone has the new list of current impact factor and five years impact then please share with me for the 2016-17 list? Many regards
05 June 2017 4,609 6 View
Can somebody please help me finding articles that are associated with longterm usage of FK506 resulting in toxicity? if animal model results of neurodegenerative disorders had toxicity it will be...
05 June 2016 10,118 2 View
i did some IHC staining on brain sections of prion infected hamsters...some slides showing overexpression and some underexpression on nuclear protein Lamin A/C? what does it mean in simple words?
03 April 2016 5,776 5 View
i have some pallets left after centrifugation of brain homogenates made in RIPA buffer. Now if i want to see NF Kb p65 nuclear translocation status in these samples, is it ok to use Urea? or any...
03 April 2016 6,806 2 View
Can anyone tell me the best protocol which can help me visualizing Nfkb P65 through confocal microscopy or any other IHC protocol in prion infected paraffin embedded section?
03 April 2016 1,444 2 View
We intend to study the interaction between peptides and polymer (like PP, PE and PS) through MD simulations using Martini force fields ( Martini 2 for PP and Martini 3 for PE, PS). We have...
08 August 2024 4,842 0 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...
07 August 2024 3,218 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...
04 August 2024 4,725 2 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...
04 August 2024 8,432 3 View
how is prion can cause disease
01 August 2024 250 1 View