i have a very simple question that if i want to see the effect of Salubrinal and tauroursodeoxycholic acid (TUDC) in growth of reproductive cells for example in growth of embryo in vitro...will it work? can i use them together in same medium? and if yes then how much the dose should be in combination use?
Dear Syed,
The following paper covers the answer to your question:
RESEARCH ARTICLE
Dichloroacetate Enhances Adriamycin-Induced Hepatoma Cell Toxicity In Vitro and In Vivo by Increasing Reactive Oxygen Species Levels
Yunhai Dai , Xiaopeng Xiong , Gang Huang , Jianjun Liu, Shile Sheng, Hongjian Wang, Wenxin Qin
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0092962
PLOS ONE
Published: April 11, 2014
DOI: 10.1371/journal.pone.0092962
Abstract
A unique bioenergetic feature of cancer, aerobic glycolysis is considered an attractive therapeutic target for cancer therapy. Recently, dichloroacetate (DCA), a small-molecule metabolic modulator, was shown to reverse the glycolytic phenotype, induce reactive oxygen species (ROS) generation, and trigger apoptosis in various tumor cells. In this work, the capacity of DCA to enhance Adriamycin (ADM) efficacy in hepatoma cells by modulating glucose metabolism and redox status was evaluated. Two human hepatoma (HCC-LM3 and SMMC-7721) and a normal liver (LO2) cell lines were treated with DCA or ADM alone, or in combination. Exposure of hepatoma cells to DCA/ADM combination resulted in significantly decreased cell viability and increased percentage of apoptotic cells as well as intracellular ROS levels, in comparison with treatment with DCA or ADM alone. However, simultaneous treatment with the thiol antioxidant N-acetylcysteine (NAC, 10 mmol/L) reduced the elevated ROS levels and protected hepatoma cells from the cytotoxic effects of DCA/ADM combination. L-buthionine-[S,R]-sulfoximine, an inhibitor of glutathione synthesis, enhanced hepatoma cell sensitivity to DCA/ADM combination. Interestingly, treatment with DCA/ADM combination did not significantly increase cytotoxicity in normal hepatocytes in comparison with the drugs administered individually. Finally, DCA reduced tumor growth and enhanced ADM efficacy on HCC-LM3 hepatoma in mice. Overall, our data suggest that DCA enhances ADM cytotoxicity in hepatoma cells by increasing intracellular ROS levels and provide a strong biochemical rationale for the use of DCA in combination with ADM for treatment of hepatoma.
Hoping this will be helpful,
Rafik
You can uses many antioxidants as you want as far there are not interactions between them. It is normal practice to add SOD and catalase together. I do not know your experimental design. There awesome antioxidants that cannot be combined because they consume the reducing power of the cell or they become toxic. This depends on the specific experimental design. The B27 supplement has several antioxidants and is designed to culture neurons without serum. I hope this helps.
In particular if your antioxidants are selective for their targets (e.g. a NOX2 inhibitor and a Nox4 inhibitor) then it is quite normal to add more than one. Same goes for most pharmacologcial approaches. It tells you whether mechanisms are acting additively or synergistically
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