What is the best protocol to visualize Nfkb-p65 in Paraffin embedded brain sections?

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2018 impact factor list?

If anyone had the new impact factor list for 2018 please share with me... Many Regards

05 June 2018 7,596 11 View

Lamin A/C more or Less in brain tissue slides. What does it indicates?

I gave prion infection to hamsters and now i did Lamin A/C stain for brain Slides? more or less expression of Lamin A/C indicates?

11 December 2017 4,457 0 View

Does anyone know why in ENDNOTE some of the references are not shown properly when you cite in paper?

I tried to cite some articles but some of them are not correct? i mean when more than three authors then in some cases it shows et al., but with many it shows full authors names no matter how many...

11 December 2017 3,590 4 View

How to differentiate the stressed mitochondria from normal mitochondrion in Transverse Electron Microscope Images?

I have TEM images of brains from infected hamsters? how can i differentiate stressed mitochondrion from normal healthy ones? any tips? Thanks

10 November 2017 7,458 4 View

Why suddenly the retraction number increased recently from many journals?

I just noticed on main page of many impact factored journals the retracted articles? What could be the cause? The ones i saw were mostly Chinese authors...

10 November 2017 6,383 0 View

Does anyone have the new impact factor list for SCI journals in year 2016-17?

If anyone has the new list of current impact factor and five years impact then please share with me for the 2016-17 list? Many regards

05 June 2017 4,609 6 View

Toxic effects of prolonged FK506 treatment in Hamsters?

Can somebody please help me finding articles that are associated with longterm usage of FK506 resulting in toxicity? if animal model results of neurodegenerative disorders had toxicity it will be...

05 June 2016 10,118 2 View

Lamin A/C overexpressed or underexpressed in brain section? what does it simply means?

i did some IHC staining on brain sections of prion infected hamsters...some slides showing overexpression and some underexpression on nuclear protein Lamin A/C? what does it mean in simple words?

03 April 2016 5,776 5 View

What will happen to NFkb p65 if i just use urea to get nuclear extract from brain homogenates?

i have some pallets left after centrifugation of brain homogenates made in RIPA buffer. Now if i want to see NF Kb p65 nuclear translocation status in these samples, is it ok to use Urea? or any...

03 April 2016 6,806 2 View

Does anyone have any idea that Calcineurin regulator unit and Catalytic Unit expression will increase if calcineurin level is higher in sample?

I wanna check Calcineurin expression Via western blot for Calcineurin A Fragment and Calcineurin B fragment...will both of these fragments expression on western blot will be high or low as the...

03 April 2016 4,608 0 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

Is there anyone with experience in TEM analysis who can assist with a manuscript for an upcoming journal?

Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...

07 August 2024 9,563 0 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

ANY free software for reconstructing neurons in the microscopic image?

Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...

04 August 2024 4,725 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

Do You Know Untreated TMJ Affects Your Brain And Entire Body?

04 August 2024 6,282 1 View

Which distribution type should I use when calculating the average particle size from TEM image? and how to calculate the error ?

average particle size calculation from TEM

04 August 2024 2,921 1 View