Primery cells are better or N2Tera cells are better for axoplasmic reticulum related calcium surge studies in axon degeneration?
I am sorry, having no answer.
If anyone had the new impact factor list for 2018 please share with me... Many Regards
05 June 2018 7,596 11 View
I gave prion infection to hamsters and now i did Lamin A/C stain for brain Slides? more or less expression of Lamin A/C indicates?
11 December 2017 4,457 0 View
I tried to cite some articles but some of them are not correct? i mean when more than three authors then in some cases it shows et al., but with many it shows full authors names no matter how many...
11 December 2017 3,590 4 View
I have TEM images of brains from infected hamsters? how can i differentiate stressed mitochondrion from normal healthy ones? any tips? Thanks
10 November 2017 7,458 4 View
I just noticed on main page of many impact factored journals the retracted articles? What could be the cause? The ones i saw were mostly Chinese authors...
10 November 2017 6,383 0 View
If anyone has the new list of current impact factor and five years impact then please share with me for the 2016-17 list? Many regards
05 June 2017 4,609 6 View
Can somebody please help me finding articles that are associated with longterm usage of FK506 resulting in toxicity? if animal model results of neurodegenerative disorders had toxicity it will be...
05 June 2016 10,118 2 View
i did some IHC staining on brain sections of prion infected hamsters...some slides showing overexpression and some underexpression on nuclear protein Lamin A/C? what does it mean in simple words?
03 April 2016 5,776 5 View
i have some pallets left after centrifugation of brain homogenates made in RIPA buffer. Now if i want to see NF Kb p65 nuclear translocation status in these samples, is it ok to use Urea? or any...
03 April 2016 6,806 2 View
Can anyone tell me the best protocol which can help me visualizing Nfkb P65 through confocal microscopy or any other IHC protocol in prion infected paraffin embedded section?
03 April 2016 1,444 2 View
How can I determine a good adhesion strength range for coatings on polymer surfaces, such as DLC on polymer substrates? Is there a specific threshold for adhesion strength (from T-peel tests)...
10 August 2024 942 3 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Vertebrates and invertebrates (e.g., arthropods: insects, crustaceans, etc.) have two different neuro-muscular systems given their differential construction: the former have an endoskeleton and...
07 August 2024 648 0 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...
06 August 2024 726 3 View