I want to see neurodegeneration in my hamsters infected with Prpsc...can anybody guide me how can i easily see either apoptotic markers in brain homogenate or any fixed tissue techniques?
thanks in advance
You can conduct a for of immunocytochemical assay or immunostaining for any specific apoptotic adaptor proteins.
You may look at presynaptic and postsynaptic markers because neurodegeneration may happen at the synaptic level and causes synaptic loss. For example, SNAP-25 is a presynaptic marker, also PSD-95 can be used as a posrtsynaptic marker. You can analyze these markers either in the brain homogenates using western blot analysis or by immunostaining of brain microsections.
good luck
Are you just looking for apoptosis specifically? PrPsc = prions, yeah?
If you're not looking specifically at axonal degeneration, it may be cheaper to go with a caspase-3 assay.
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