How to differentiate the stressed mitochondria from normal mitochondrion in Transverse Electron Microscope Images?

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2018 impact factor list?

If anyone had the new impact factor list for 2018 please share with me... Many Regards

05 June 2018 7,596 11 View

Lamin A/C more or Less in brain tissue slides. What does it indicates?

I gave prion infection to hamsters and now i did Lamin A/C stain for brain Slides? more or less expression of Lamin A/C indicates?

11 December 2017 4,457 0 View

Does anyone know why in ENDNOTE some of the references are not shown properly when you cite in paper?

I tried to cite some articles but some of them are not correct? i mean when more than three authors then in some cases it shows et al., but with many it shows full authors names no matter how many...

11 December 2017 3,590 4 View

Why suddenly the retraction number increased recently from many journals?

I just noticed on main page of many impact factored journals the retracted articles? What could be the cause? The ones i saw were mostly Chinese authors...

10 November 2017 6,383 0 View

Does anyone have the new impact factor list for SCI journals in year 2016-17?

If anyone has the new list of current impact factor and five years impact then please share with me for the 2016-17 list? Many regards

05 June 2017 4,609 6 View

Toxic effects of prolonged FK506 treatment in Hamsters?

Can somebody please help me finding articles that are associated with longterm usage of FK506 resulting in toxicity? if animal model results of neurodegenerative disorders had toxicity it will be...

05 June 2016 10,118 2 View

Lamin A/C overexpressed or underexpressed in brain section? what does it simply means?

i did some IHC staining on brain sections of prion infected hamsters...some slides showing overexpression and some underexpression on nuclear protein Lamin A/C? what does it mean in simple words?

03 April 2016 5,776 5 View

What will happen to NFkb p65 if i just use urea to get nuclear extract from brain homogenates?

i have some pallets left after centrifugation of brain homogenates made in RIPA buffer. Now if i want to see NF Kb p65 nuclear translocation status in these samples, is it ok to use Urea? or any...

03 April 2016 6,806 2 View

What is the best protocol to visualize Nfkb-p65 in Paraffin embedded brain sections?

Can anyone tell me the best protocol which can help me visualizing Nfkb P65 through confocal microscopy or any other IHC protocol in prion infected paraffin embedded section?

03 April 2016 1,444 2 View

Does anyone have any idea that Calcineurin regulator unit and Catalytic Unit expression will increase if calcineurin level is higher in sample?

I wanna check Calcineurin expression Via western blot for Calcineurin A Fragment and Calcineurin B fragment...will both of these fragments expression on western blot will be high or low as the...

03 April 2016 4,608 0 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

Hello researchers Is this a random laser or just fluorescence?

I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.

09 August 2024 9,894 2 View

1. If I can quantize the atom using this hyperbolic spiral and classical physics, could nature do the same?

If we map as a continuous motion an ionising electron (beginning its journey at n=1) in an H atom, a specific hyperbolic spiral appears (see animation). When we solve this spiral formula, we find...

07 August 2024 5,343 2 View

Is there anyone with experience in TEM analysis who can assist with a manuscript for an upcoming journal?

Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...

07 August 2024 9,563 0 View

How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under op microscop?

How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under optical microscope?

07 August 2024 2,132 3 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Which distribution type should I use when calculating the average particle size from TEM image? and how to calculate the error ?

average particle size calculation from TEM

04 August 2024 2,921 1 View

Dirty and clean?

Hi everyone I need a file with a dirty and clean potato image

04 August 2024 7,199 4 View

Why reactivity isn't increased with more empty spots in valence shell?

If from a geometric perspective the non-halogens, non-noble gases have more empty spots in their valence shell, and the filling/exiting of any of the empty spots in the shell constitutes a...

03 August 2024 4,787 2 View

Why is the molecule's orientation with an electric field affect polarizability?

Why is the molecule's orientation with an electric field affect polarizability? Electrons are diffuse enough to be independent with respect to orientation and effect of electric field on...

03 August 2024 7,843 1 View

Syed Zahid Ali Shah

sorry i mean transmission electron microscope...lol

Brent E Gowen

Have a look at the normal and stressed mitochondria images/text from; PNAS 112 (33); 10162–10168.

Daniela Rodler

Stress frequentle cause an increase in size and cristae number of mitochondria. Enlargement of mitochondria can be caused by various l conditions and can lead to the formation of megamitochondria.

Kind regards,

Daniela

thanks all