How to differentiate the stressed mitochondria from normal mitochondrion in Transverse Electron Microscope Images?

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2018 impact factor list?

If anyone had the new impact factor list for 2018 please share with me... Many Regards

05 June 2018 7,410 11 View

Lamin A/C more or Less in brain tissue slides. What does it indicates?

I gave prion infection to hamsters and now i did Lamin A/C stain for brain Slides? more or less expression of Lamin A/C indicates?

11 December 2017 4,269 0 View

Does anyone know why in ENDNOTE some of the references are not shown properly when you cite in paper?

I tried to cite some articles but some of them are not correct? i mean when more than three authors then in some cases it shows et al., but with many it shows full authors names no matter how many...

11 December 2017 3,382 4 View

Why suddenly the retraction number increased recently from many journals?

I just noticed on main page of many impact factored journals the retracted articles? What could be the cause? The ones i saw were mostly Chinese authors...

10 November 2017 6,217 0 View

Does anyone have the new impact factor list for SCI journals in year 2016-17?

If anyone has the new list of current impact factor and five years impact then please share with me for the 2016-17 list? Many regards

05 June 2017 4,446 6 View

Toxic effects of prolonged FK506 treatment in Hamsters?

Can somebody please help me finding articles that are associated with longterm usage of FK506 resulting in toxicity? if animal model results of neurodegenerative disorders had toxicity it will be...

05 June 2016 9,945 2 View

Lamin A/C overexpressed or underexpressed in brain section? what does it simply means?

i did some IHC staining on brain sections of prion infected hamsters...some slides showing overexpression and some underexpression on nuclear protein Lamin A/C? what does it mean in simple words?

03 April 2016 5,600 5 View

What will happen to NFkb p65 if i just use urea to get nuclear extract from brain homogenates?

i have some pallets left after centrifugation of brain homogenates made in RIPA buffer. Now if i want to see NF Kb p65 nuclear translocation status in these samples, is it ok to use Urea? or any...

03 April 2016 6,625 2 View

What is the best protocol to visualize Nfkb-p65 in Paraffin embedded brain sections?

Can anyone tell me the best protocol which can help me visualizing Nfkb P65 through confocal microscopy or any other IHC protocol in prion infected paraffin embedded section?

03 April 2016 1,261 2 View

Does anyone have any idea that Calcineurin regulator unit and Catalytic Unit expression will increase if calcineurin level is higher in sample?

I wanna check Calcineurin expression Via western blot for Calcineurin A Fragment and Calcineurin B fragment...will both of these fragments expression on western blot will be high or low as the...

03 April 2016 4,472 0 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

How to find the mode of classified ocean colour images?

Hello, I have classified 10 S3 OLCI images from the same area (althouth not the same size and/or quadrant) and I would like to do a final image using the mode of the pixel of these 10 images. I...

01 March 2021 9,874 3 View

Syed Zahid Ali Shah

sorry i mean transmission electron microscope...lol

Brent E Gowen

Have a look at the normal and stressed mitochondria images/text from; PNAS 112 (33); 10162–10168.

Daniela Rodler

Stress frequentle cause an increase in size and cristae number of mitochondria. Enlargement of mitochondria can be caused by various l conditions and can lead to the formation of megamitochondria.

Kind regards,

Daniela

thanks all