Protease inhibitor coctail quantity in brain homogenate?

More Syed Zahid Ali Shah's questions See All

2018 impact factor list?

If anyone had the new impact factor list for 2018 please share with me... Many Regards

05 June 2018 7,410 11 View

Lamin A/C more or Less in brain tissue slides. What does it indicates?

I gave prion infection to hamsters and now i did Lamin A/C stain for brain Slides? more or less expression of Lamin A/C indicates?

11 December 2017 4,269 0 View

Does anyone know why in ENDNOTE some of the references are not shown properly when you cite in paper?

I tried to cite some articles but some of them are not correct? i mean when more than three authors then in some cases it shows et al., but with many it shows full authors names no matter how many...

11 December 2017 3,382 4 View

Why suddenly the retraction number increased recently from many journals?

I just noticed on main page of many impact factored journals the retracted articles? What could be the cause? The ones i saw were mostly Chinese authors...

10 November 2017 6,217 0 View

How to differentiate the stressed mitochondria from normal mitochondrion in Transverse Electron Microscope Images?

I have TEM images of brains from infected hamsters? how can i differentiate stressed mitochondrion from normal healthy ones? any tips? Thanks

10 November 2017 7,269 4 View

Does anyone have the new impact factor list for SCI journals in year 2016-17?

If anyone has the new list of current impact factor and five years impact then please share with me for the 2016-17 list? Many regards

05 June 2017 4,446 6 View

Toxic effects of prolonged FK506 treatment in Hamsters?

Can somebody please help me finding articles that are associated with longterm usage of FK506 resulting in toxicity? if animal model results of neurodegenerative disorders had toxicity it will be...

05 June 2016 9,945 2 View

Lamin A/C overexpressed or underexpressed in brain section? what does it simply means?

i did some IHC staining on brain sections of prion infected hamsters...some slides showing overexpression and some underexpression on nuclear protein Lamin A/C? what does it mean in simple words?

03 April 2016 5,600 5 View

What will happen to NFkb p65 if i just use urea to get nuclear extract from brain homogenates?

i have some pallets left after centrifugation of brain homogenates made in RIPA buffer. Now if i want to see NF Kb p65 nuclear translocation status in these samples, is it ok to use Urea? or any...

03 April 2016 6,625 2 View

What is the best protocol to visualize Nfkb-p65 in Paraffin embedded brain sections?

Can anyone tell me the best protocol which can help me visualizing Nfkb P65 through confocal microscopy or any other IHC protocol in prion infected paraffin embedded section?

03 April 2016 1,261 2 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

What element is driving the different slop in the low frequency tail?

My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...

01 March 2021 6,474 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

What kind of food stuff increase the potentiality of human brain?

There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...

01 March 2021 831 2 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Syed Zahid Ali Shah

10 microlitres per ml? am i right?

Sadeeq Ur Rahman

If it is 100x then fir 100 ul homog u add 1 ul

thanks dr sb for response so according to your answer i was right...because in one ml 10 microlitres are same as what u said...

thanks again

Frédérique Masmejean

You're right

yes= 1000/100=10

thanks to both of u