Funny thread, since 3 weeks everyday somebody write which kit is the best, we should make an statistic and write a paper about it ;-)

I used RNA purification Kit from Fermentas, for mammalian cell lines and it works very good !!

1)Invitrogen

2)Qiagen

3)Etc...

Hi Danielle,

for the RNA extraction from my aphids I use this kit: Master Pure RNA isolation kit from Epicentre (there are some more for RNA extraction depending on your material: http://www.biozym.com/DesktopModules/WebShop/shopdisplaycategories.aspx?id=163).

The kit is not expensive and my downstream applications (RT-PCR) work very well with it (and it's always enough RNA even from a small insect).

And you don't need much time for it!

Cheers, Nadine

Hi Danielle, I'm dealing with cancer and normal cells. Until now i've used more than 10 commercial available kits. The RNeasy mini kit my QIAGEN is an easy one, reliable and very easy to use. The amoun of RNA that is produced is enough for further applications and has also a good quality and purity.

Hello Danielle,

I use also Qiagen for cells and the kit from epicentre for isolation of RNA from blood.

I also used RNeasy from Qiagen for RNA extraction from human cells or tissues. The RNA is always good quality and purity (and it works even starting from less than 1 million cells)

I usually use Qiagen kit for RNA isolation.

I prefer Qiagen kit!

I have used both Qiagen and Fermentas for RNA isolation from Gram+ve bacteria (Streptomyces) and Qiagen yields better results.

RNeasy from Qiagen is a very good one

Qiagen is good but it does not retain miRNA fraction. If you are interested also in miRNAs, you should move to TRIzol or mirVana kit from Ambion. I have been using mirVanaand it works very well for cell lines (RIN higher than 9) with a good recovery of miRNA (higher than 0.5%)

Qiagen kit give the best results compared with promega, roche and fermentas.

I like PoliT-magnetic beads from Dynal, it is for mRNA. It is faster than a spin-column and works really well with low amounts of cells. Besides Dynal has specific kits for different kind of cells. I highly recommend it.

Used Qiagen for years and never had problems (RT-PCR for semi-quantitative analysis, gene cloning and real time PCR). I have just ordered a kit from Norgen Biotek (Canadian company, a lot cheaper than Qiagen) and allows you to purify RNA and protein at the same time from the same sample (important with a few mg patient sample). Haven't tested yet but as usual according to all sales reps it is better - only believe once tested.

we use RNeasy kits of Qiagen.

Qiagen kits gives good results

Nowadays RNA extraction kits are all pretty good. Qiagen has always been the "tall poppy" although pricey. RNA NuceloSpin II is, as far as I am concerned, as good or better as Qiagen. Either way, you won't go wrong with one or the other. In my experience, Roche is also good and Promega is just average. Good luck!

I also use the RNeasy to isolate total RNA from bacteria such as Pseudomonas aeruginosa.

We have ued Qiagen for a long time, now we use Fermentas kits which work as fine for only a fraction of the Qiagen price.

For extract total RNA from cell culture, I recommend Qiagen or Illustra (GE healthcare).

I trust Ambion. Either miRVana or RiboPure work good

Oiagen RNeasy Mini Kit is the best choice.

We use QIAGEN, although Zymogen are cheaper and come highly recommended.

Qiagen RNeasy mini kit is good and easy to use, for higher yields you can also use Fermentas GeneJet RNA purification kit.

We tried two different kits from Qiagen and Sigma. Both worked very well, however Sigma kits are cheaper.

Dear Danielle,

Hi. Regarding to question I am afraid to say that the best general kit for RNA isolation does not exist. Choice of kits is first depending on downstream assays (Northern blot, microarrays, Real time PCR from total RNA, mRNA or microRNA). In general there are 3 approaches

1. Phenol extraction based methods (such as Trizol or Tri pure) which are cheap and are ok for microarray analysis and isolating total RNA and microRNAs . However, disadvantage remains contamination with proteins or genomic DNA and or phenol and other solvent residues inhibiting cDNA or DNAsynthesis related enzymes such as Taq polymerases.

2. Affinity column based methods such as Qiagen depending type of cell or tissues, Highpure from Roche or nucleospin (if you have not much cells; 4000 cells and more). The quality of RNA is fair and RNA is clean however, it cannot always used for some applications e.g efficient microRNAs isolation and yield is lower than other two methods.

3. Magnetic beads based methods such as Roche Magnapure which needs robot to isolate RNA or dynal from Life Sciences. This method ensures best quality and yield for mRNA or total RNA isolations. However, the price is the most disadvantage of using magnetic beads.

I hope you can use this information for choice of best working method for your specific research question

Hello..

As far as my knowledge and research experience you can relie on RNA NuceloSpin II kit.

In my hands the qiagen RNeasy Plus kits can't be beat.

We use the Qiagen columns on aday to day basis with good yields and high quality RNA. the maxi kit is suitable for arrays. Note that they have a special procedure to deal with salts if you encounter such problems

I have tried several along years and was always most satisfied with Qiagen's RNeasy (also for microarrays and RNAseq) regarding the RNA quality, yealds, speed, and price.

See: http://www.biomedcentral.com/1756-0500/4/3

We made best experiences with Trizol/Phenol/Chloroform extraction. This RNA was used for library construction and qPCRs and worked nicely.

QIAGEN's RNeasy is very good but Roche HighPure is also good and cheaper. Although, we have higher yields with Trizol/Phenol/Chloroform plus glycogen and you can storage your whole experiment samples.

Qiagen is the best name

Hi Danielle,

I believe it depends from where you will isolate RNA. For example a nice kit for RNA tissue extraction is Qiagen, for cells BioRad is very good and practical, and less time consuming.

A very very good kit for RNA extraction from frozen tissue in PureLink® Kits by invitrogen or BioRad

Qiagen's RNeasy kits are great for most cases. Its easy to work with and the yield is excellent for most tissues. Unless you have an especial need that requires a certain type of kit. Good Luck!

Hi Danielle

We've tried out many protocol's in our lab (lots of kits and lots of home made protocols). If you're just doing something simple (eg qPCR from millions of cells) then I don't think it really matters what you go for (we generally go with Qiagen.. however we have no rationale for choosing this kit as all the kits we've tried work well in this situation). For more complex experiments (eg qPCR from a single cell or very small numbers of cells, or from sorted primary material) all the kits seem to be as bad as each other, but in this instance we tend to favour PicoPure and Qiagen's RNAeasy micro kit.

Best of luck

Al

TRIzol®, PureLink®, MagMAX™, mirVana™, and Dynabeads® RNA technologies that offer the high RNA yield and quality.

Below link provides the information.

http://www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/DNA-RNA-Purification-Analysis/rna-extraction.html

BioRad is very good

I usually use Macherey-Nagel kits, but they are particularized, for plant tissue for example. “Total RNA Purification from Plant”-kit ever gives good result. I think others kits of this firm also not bad

Dear Danielle,

Most of the kits are based on phenol chlorophorme extraction. Chlorophorm is a mutagenic/cancerogenic agent. Therefore, try the kits where you don't have to use chlorophore. I recomend you two good and cheap kits from A&A Biotechnology

1. Total RNA Mini Plus link: http://www.shop-aabiot.home.pl/en_GB/p/Total-RNA-Mini-Plus-25-isolations/225

2. The second kit Total RNA Mini Plus Concentrator allows to do the extration from the very small samples and elute RNA in 15 ul. See link: http://www.shop-aabiot.home.pl/en_GB/p/Total-RNA-Mini-Plus-Concentrator-25-isolations/233

Best regards,

Slawomir Dabrowski

Qiagen, RNAeasy...

I have routinely used RNeasy kit from Qiagen and have never had any problems. The yield and quality are good and the kit is trustworthy. All the best.

Himedia's convenient kits for isolating total RNA and mRNA from diverse sample types. it is basic and also the best...... for every .....

Maybe it's enough to recommend kits?! All of you named a lot of different kits. Now it's Danielles choice in picking one... :-)

Cheers, Nadine

Hi Danielle, there are many smart kits available in the market and all have their own advantages. I have good experience with miRCURY RNA isolation kit from EXIQON, as it offers many key benefits (you can visit www.exiqon.com)

I also agree, that all the kits mentioned work very well if you don't have any special problems like high lipid content. But there are kits from qiagen, that isolate RNA, DNA and protein at the same time. It's column based, too. I don't know if you need DNA and protein for your analyses, but if so you only need one probe for all the three things, and they correspond to each other! In my opinion this is a great plus!!

Good luck!

I've tried them all. Roche is the best.

I have used many RNA extraction kits, and the ones I prefer are the ones that Zymo Research has. They are inexpensive and give really good quality RNA. No phenol/chloroform extraction, just a lysis buffer, spin column, and wash buffer (for their Quick-RNA kits). You get pure RNA in about 10-15 minutes. There are a variety of kits to suit your needs: http://www.zymoresearch.com/rna-purification/total-rna-purification

I have had no problem with any of the QIAGEN RNeasy kits. Recently used the Micro RNeasy kit to extract RNA from a very small number of cells which resulted in high quality RNA. Have since used that RNA for microarray analysis and it was very high quality.

I used the Agilent bioanalyser to test the quality and look for high RNA integrity number (RIN).

For up to one million cells though, the standard RNeasy mini kit (spin columns) using the QIAShredder columns work perfectly well.

You can use Qiagen's RNA Isolation kit. It's the best.

Kits are a waste of money, I have really good experiences with home-made TriZOL, but recently I started using a method that doesn't even use organics or BME. It works great for small amounts of tissue, and the yield is very good, so it is perfect for qPCR.

Try out this method by Oñate-Sánchez* and Vicente-Carbajosa:

http://www.biomedcentral.com/1756-0500/1/93

although most of the time using trosol is recommended ,because of high yield of RNA,based on my own experience i recommend you to use QIAGEN kit,which an easy to use one and by high concentration resulted RNA.

I have tried Qiagen, Roche and Invitrogen PureLink RNA isolation kits for whole blood, I think all of them give quite similar yield of RNA and not much difference in the A260/280 as well (though I haven't run any gel for the quality check). Invitrogen is the cheapest which use lysis buffer, spin column and wash buffer, very user friendly. Qiagen is good as well but quite expensive.

I have used QIAGEN RNeasy for viral RNA extraction, together with cDNA and found good results , Invitrogen and Roche kits are also recommendable.

I like the 5prime kit:

http://www.5prime.com/products/nucleic-acid-purification/rna-purification/perfectpure-rna-cultured-cell-kit.aspx

But of course there are many other good kits (e.g. Qiagen).

Just a general remark - before choosing a kit, your sample conditions are more important - high RNase concentration, number of cells etc.

I am with Ujjawal Sharma, TRI-reagent is best!!!

I recently used the ollowing Norgen kit.

Total RNA Purification Kit Total

Cat # 17200

I got very good RNA yield and great quality.

Qiagen RNeasy kit works very well in our hands with reproducible results

RNAse inhibitors like RNAsin are recommended

Depending on your starting material, Qiagen kits are pretty good.

Hi from Turkey;

i used almost all RNA isolation kits but i can honestly say that QIAGENE RNA isolation kit is the best.. thy is you will like..

Mirvana kit is very good. Please follow the whole RNA isolaion protocol.

Really depends on what you want to isolate. I find that with the Trizol/Qiazol series of kits it works pretty well. However, you have to dispose of the chemicals properly due to the presence of various organic solvents in the solutions such as phenol.

We use RNA extraction kit with column from Macherey-Nagel. Nucleospin.

It works very well and it is done in 1h.

Ribopure from Applied Biosystem

In terms of not compromised RNA quality but really low price one of the best are:

1. A&A Biotechnology (http://www.aabiot.home.pl/Main/RNAEN/RNA_isolation/RNA_isolation.html)

2. Novazym Polska (http://www.novazym.ehost.pl/esklep4/rna-purification-c-55_12.html?language=en)

3. DNA Gdansk (http://www.dnagdansk.com/en/category,40/rna-purification-kits/)

I had good results with column based kits from all these companies. However I work now with potato tubers, and so far the best for that material is manual isolation using modified chomczynski reagent (RNA-3-zone from Novazym Polska).

Rnazol RT from Sigma

Used TRI, Trizol a lot before. Now, switch to use RNzol RT from MRC. No more chloroform disposal.

Aurum Total RNA Fatty and Fibrous Tissue Kit from biorad

Qiagen Kit is best .

Depends on what you want to isolate.. in my experiment Trizol and Qiagen kit is the best..

I recommended qiagen kit itis wouderful

Funny thread, since 3 weeks everyday somebody write which kit is the best, we should make an statistic and write a paper about it ;-)

@Sven: Thank you! I thought the same. Everybody is recommending the same kit. Why??? Why are people not voting up the answer if they want to recommend the same one?

Qiagen RNA isolation kits work very well, but it depends on your application. Are you going to run rT-PCR or are you performing Northern Blot analysis. Each kit has their limitations. If you are giong to be running rT-PCR, I highly recommend Qiagen RNA-easy Kits...however, if you're going to be running Northern Blot Analysis then Chloroform Phenol Extractions are still the Gold-Standard

You can try so hot phenol acid/chloroform (4x15min at 65°) it works and it's cheaper! ;-)

@Ashish: Didn't you check the answers before?!

Please close this thread!!!!!

The Roche RNA isolation kit is good because it comes with RNAse-free DNAse included and it is considerably cheaper than Qiagen RNAEasy. Most kits these days are equivalent.

We tried a few cheaper kits in our lab for RNA isolation. Generally, it is better to stick with the tried-and-tested to avoid variable results. We now use QIAGEN's kit for the majority of RNA extractions, however Agilent produces a good kits for isolation of RNA from brain tissue (the QIAGEN kits does not manage this quite as well).

I used both Qiagen and Roche. Both work fine, but if you want to keep the supernatant (the first flow-through) for ELISA protein assay I recommend Qiagen, it does not interfere so much with the kits available on market.

I always use phenol based extraction (TRI, Trizol or RNAzol RT) to get total RNA, which includes microRNA as well. It really depends on what downstream experiments you plan to do. If you just need it for checking gene (not small RNA) expression, column-based method like Qiagen RNAeasy kit is enough.

There are a few things to consider, first money - if you don't have enough money, just use conventional method. Second is the nature of your work after getting RNA.

We have used RNAeasy / RNAlater from Qiagen with good results. Much less of a mess than phenol chloroform extractions.

depends on your sample. I still prefer to use Trizol followed by a column clean with either an RNeasy (Qiagen) or similar kit. If you want miRNA make sure the column will catch small species.

QIAGEN.....RNeasy is best.

What are the best primers for plasmodium species id

Am done with first year masters in molecular biology and i want to do research in maize fungal infection during storage, how can i secure funding

Defends a lot on your funds of course but have used QIAGEN..RNeasy for cultured cells with good yield

I think Qiagen is mentioned 40 times. If you have something new to mention then it's okay otherwise it's a waste of time!