Luciano G. Martelotto

5 Questions 48 Answers 0 Followers

Questions related from Luciano G. Martelotto

Did anyone attempt to shear the cfDNA prior to library prep?

CfDNA from cancer patients ofter show a trio of peaks products of mono-, di- and tri-nucleosome fragments. We usually repair and make liberaries directly without enriching for smallest peak (we...

04 April 2017 8,076 0 View

Can I take the standard deviations of average DCt?

I'm doing some qRTPCR data analysis on tumour samples and using macthed "normal" tissue as a control. Each normal and tumour was run in triplicate (technical). Now we want to use all the normal as...

06 June 2014 681 4 View

Is e-cadherin expressed (protein level) in HEK293 cells?

I read several papers and I found contrasting information regarding the expression of E-cadherin in HEK293 cells. I haven't run a WB as yet, but any personal experience will help.

04 April 2014 5,476 1 View

Input control ChIP-seq?

How much chromatin Input control is used for Input library preparation for Sequencing?

08 August 2013 5,432 0 View

Fluorescence fading after transfection?

I'm having troubles maintaining my fluorescence, either RFPtag or ZsGreen, expression after a few days under selection with G418. I'm working with MCF7, T47D and NIH3T3. They transfect ok...not as...

07 July 2013 8,893 2 View