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Questions related from Luciano G. Martelotto
CfDNA from cancer patients ofter show a trio of peaks products of mono-, di- and tri-nucleosome fragments. We usually repair and make liberaries directly without enriching for smallest peak (we...
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I'm doing some qRTPCR data analysis on tumour samples and using macthed "normal" tissue as a control. Each normal and tumour was run in triplicate (technical). Now we want to use all the normal as...
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I read several papers and I found contrasting information regarding the expression of E-cadherin in HEK293 cells. I haven't run a WB as yet, but any personal experience will help.
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How much chromatin Input control is used for Input library preparation for Sequencing?
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I'm having troubles maintaining my fluorescence, either RFPtag or ZsGreen, expression after a few days under selection with G418. I'm working with MCF7, T47D and NIH3T3. They transfect ok...not as...
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