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Questions related from Budak Drop out
I want to publish manuscript related to protein modeling with low impact factor,indexed by ISI, scopus..
02 February 2015 8,131 6 View
Can anyone give comments for this DOPE profile? Thank you.
01 January 2015 5,541 1 View
I cant open it after clicking its web link on google.
12 December 2014 6,920 0 View
I've got problems during filling the submission form for protein sequences. After filling the form and pressed the submit button, it stated as "can't open file". How can I solve this? thanks.
12 December 2014 3,189 6 View
I am a novice user for using protein bioinformatics tools. I don't know the clear path for analyzing my amino acid sequences of interest for modelling 3-D structure. What is the initial step of...
12 December 2014 8,621 3 View
Floral volatiles from biosynthesis to function
12 December 2014 7,499 0 View
My current files for modeller 9.14 (Mod9.14) were all in notepad, except for protein sequences in pdb format. I dont know how to change my files to .ali and .py format. Any suggestion?
12 December 2014 9,574 2 View
I eluted purified his-tag protein in 200 ul of elution buffer containing 500 mM imidazole. Then, I want to use it for SDS-PAGE to confirm the presence of specific his-tag protein band on gel. What...
08 August 2013 6,006 12 View
Is it necessary to put any liquid on top of TEMED-treated separating gel mixture between spacer plates with 1.0 mm Integrated spacers in closed casting frame?If yes, what type of liquid...
07 July 2013 261 46 View
Is it ok if I prolong run time for sds-page at 120 volt and very low current (1-2 mA) ?
07 July 2013 5,486 15 View
What factors are affecting this condition? separating gel- 12% stacking gel- 4% Even my protein marker did not show any bands in separating gel after staining with coomassie blue and destaining...
07 July 2013 4,633 28 View
Antineoplaston therapy
07 July 2013 5,814 22 View
Can anyone let me know the websites about cancer pathway? So far, I found only two well-updated info about cancer pathway deposited to the database such as NetPath and cancer cell map. Thanks.
07 July 2013 2,466 2 View
Is it true that the use of H2 blocker, proton pump inhibitors and other antacids can lead to the progression of recurring heartburn? The effect of reducing stomach acid can also make gastrin to be...
07 July 2013 2,106 5 View
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07 July 2013 1,988 4 View
Which method is suitable for precipitating protein from yeast cell lysate? From my own search, I found that the method of precipitating the protein is not a single method because there are a few...
07 July 2013 3,669 6 View
Is it possible to run protein samples on agarose gel only?My predicted size of desired proteins is around 30-60 kDa. How much the concentration of agarose should be used for separating protein...
07 July 2013 9,287 10 View
What is the suitable temperature for keeping yeast liquid culture after incubating at 30c, 250 rpm for overnight? The type of yeast is saccharomyces cerevisiae. I plan to keep it first for a few...
07 July 2013 5,529 11 View
I noticed that oxygen therapy can help patients and even a healthy individual to get an optimum health state by the way diminishing the probability of oxygen deprivation. Oxygen deprivation is a...
07 July 2013 8,333 13 View
Any available software for this purpose?
07 July 2013 9,270 2 View
Can anyone explain it?Is it similar to modern medicine?If not, what is the difference between both?
07 July 2013 9,215 1 View
can it be selective for YPD agar to have an antibiotic such as ampicillin in order to grow saccharomyces cerevisiae cells that may contain protein expression vector (pklac2) which has amp genes?...
06 June 2013 612 14 View
Is it good to use for western blotting? Can anyone state any journal that mentioned this kit for western blot analysis in the experiment?
06 June 2013 2,639 1 View
Has anyone used rabbit primary antibody for western blot? I am going to try to blot my protein of interest using a western blot kit, namely Western Max tm HRP chromogenic detection kit.The kit is...
04 April 2013 3,971 15 View
The gap width of cuvette I use is 0.4 cm. Can I use this cuvette for yeast and bacterial cells? Can anyone give me the protocol of using this cuvette with bio-rad micropulser electroporator?
04 April 2013 6,658 4 View
How to design his-tag primer? Do I need to design it at both directions, forward and reverse? In my case, I have specific primer (F&R) for specific full-length gene. The problem happened when I...
04 April 2013 6,820 7 View
Do anyone have an experience in setting up PCR with his-tag primer? My his-tag primer was designed at N-terminal (forward only).This is the conditions of my his-tag primer as shown below:- Tm=...
04 April 2013 5,493 13 View
I got a single band which represented the actual size for the DNA of interest. I want to purify it before ligating with a cloning vector. Before ligation, I also want to add an A-tail. Which...
04 April 2013 4,293 6 View
Which type of E. coli can be used as an expression vector for expressing heterologous protein of interest from plant-based genes?
04 April 2013 9,832 4 View
Can it be used for e. coli transformation if JM109 stock is expired? Is there any chance of even one single colony growing on lb plate treated with 100 mg/ml ampicillin by using expired JM109 stock?
04 April 2013 2,297 6 View
How to apply metabolomics for research of plant science especially in fragrance studies? Can anyone outline the steps of plant metabolomics properly and easy to be understood?
03 March 2013 9,620 2 View
Which one is good for treating RNase? Can anyone state reasons why the application of using DEPC is more common than DMPC for RNA work?
03 March 2013 3,325 2 View
What is the factor which causes the Taq polymerase enzyme to stop in a PCR reaction?
03 March 2013 4,694 10 View
A team of cancer researchers at Cold Spring Harbor Laboratory (CSHL) has solved the mystery of how one of the most powerful of the body’s natural tumour-suppressing proteins, called Chd5, exerts...
01 January 2013 8,791 0 View
