Nasibeh Daneshvar

7 Questions 13 Answers 0 Followers

Questions related from Nasibeh Daneshvar

Best media for MEF feeder layer cells

I thaw a vial of MEF feeder layer cells of irradiated mouse embryonic fibroblast (iMEF) feeder cells from CF1 mice (GlobalStem), and culture in high glucose DMEM supplemented with 10% serum,...

04 April 2013 5,421 22 View

Matrigel alternatives

One of our suppliers introduced me to Cultrex® BME PathClear and Geltrex® Matrix as the alternatives to matrigel. I was wondering as if anybody has experience of using these products?

03 March 2013 6,234 4 View

Which product (kit) is suitable for AP staining?

Ap staining kit

02 February 2013 3,864 10 View

Which brand of antibody is best for using in flow cytometry and ICC?

I need to do flow cytometry, I was wondering which brand has the best primary and conjucated secondary antibodies for a robust detection.

02 February 2013 1,001 9 View

Indirect flow cytometry (FACS) protocol

What is the best flow cytometry protocol using a primary antibody and conjugated secondary antibody?

02 February 2013 8,920 1 View

Sodium azide role in flow cytometry

What is the role of soduim azide in the flow cytometry procedure and is there any other chemical to replace it?

02 February 2013 9,668 5 View

What are the passage-dependent effects on cell cultures?

Cell culture

01 January 2013 694 3 View